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Statements

Subject Item
n2:RIV%2F62157124%3A16170%2F12%3A43871654%21RIV13-GA0-16170___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
This paper describes the partial results of an echocardiographic study in sixty outbreed Wistar rats. Animals of parity sex ratio were chosen for the experiment. The animals were grown up during the observation period (the minimum weight was 220 g; the maximum weight was 909 g) and were then sequentially anaesthetised (2-2.5% of isoflurane, 3 l/min O2). The second, fourth and fifth examinations were performed under anaesthesia maintained by intramuscular injections with diazepam (2 mg/kg), xylazine (5 mg/kg) and ketamine (35 mg/kg). Transthoracal examination was done using the SonoSite Titan echo system (SonoSite Ltd.) with a microconvex transducer C11 (8-5 MHz). M-mode (according to the leading-edge method of American Society of Echocardiography) echocardiography data were acquired at the papillary muscle: systolic and diastolic interventricular septum (IVSs, d) and left vetricular posterior wall (LVPWs, d) thickness, systolic and diastolic left ventricular dimension (LVDs, d), aorta (Ao) and left atrium (LA) dimensions. According to standard formulas, the following parameters were obtained: ejection fraction (EF), cardiac output (CO), stroke volume (SV), left ventricle end systolic volume (LVESV), left ventricle end diastolic volume (LVEDV), interventricular septum fractional thickening (IVSFT), left ventricular dimension fraction shortening (LVDFS), and left ventricle posterior wall fraction thickening (LVPWFS). In our study we performed 300 examinations both in male and female Wistar rats of various body weights and calculated regression equations to predict expected normal echocardiographic parameters for rats with arbitrary weights. The rats were examined by an echo scan. The first and third examinations were performed during monoanaesthesia induced by inhalation of isoflurane. Correlations, with one exception (LVDs), were very close, which means that the results of the calculations based on regression equations are very reliable. This paper describes the partial results of an echocardiographic study in sixty outbreed Wistar rats. Animals of parity sex ratio were chosen for the experiment. The animals were grown up during the observation period (the minimum weight was 220 g; the maximum weight was 909 g) and were then sequentially anaesthetised (2-2.5% of isoflurane, 3 l/min O2). The second, fourth and fifth examinations were performed under anaesthesia maintained by intramuscular injections with diazepam (2 mg/kg), xylazine (5 mg/kg) and ketamine (35 mg/kg). Transthoracal examination was done using the SonoSite Titan echo system (SonoSite Ltd.) with a microconvex transducer C11 (8-5 MHz). M-mode (according to the leading-edge method of American Society of Echocardiography) echocardiography data were acquired at the papillary muscle: systolic and diastolic interventricular septum (IVSs, d) and left vetricular posterior wall (LVPWs, d) thickness, systolic and diastolic left ventricular dimension (LVDs, d), aorta (Ao) and left atrium (LA) dimensions. According to standard formulas, the following parameters were obtained: ejection fraction (EF), cardiac output (CO), stroke volume (SV), left ventricle end systolic volume (LVESV), left ventricle end diastolic volume (LVEDV), interventricular septum fractional thickening (IVSFT), left ventricular dimension fraction shortening (LVDFS), and left ventricle posterior wall fraction thickening (LVPWFS). In our study we performed 300 examinations both in male and female Wistar rats of various body weights and calculated regression equations to predict expected normal echocardiographic parameters for rats with arbitrary weights. The rats were examined by an echo scan. The first and third examinations were performed during monoanaesthesia induced by inhalation of isoflurane. Correlations, with one exception (LVDs), were very close, which means that the results of the calculations based on regression equations are very reliable.
dcterms:title
Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats. Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats.
skos:prefLabel
Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats. Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats.
skos:notation
RIV/62157124:16170/12:43871654!RIV13-GA0-16170___
n10:predkladatel
n11:orjk%3A16170
n3:aktivita
n12:P n12:I
n3:aktivity
I, P(ED1.100/02/0123), P(GP305/08/P297)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n4:9388583 n4:1031279 n4:7573235 n4:6231659
n3:druhVysledku
n9:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
124535
n3:idVysledku
RIV/62157124:16170/12:43871654
n3:jazykVysledku
n18:eng
n3:klicovaSlova
ketamine; xylazine; diazepam; isoflurane; Wistar; M-mode; echocardiography; rat
n3:klicoveSlovo
n8:M-mode n8:ketamine n8:diazepam n8:Wistar n8:rat n8:isoflurane n8:xylazine n8:echocardiography
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[ED0137E2D773]
n3:nazevZdroje
Veterinární medicína
n3:obor
n17:GJ
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:projekt
n14:GP305%2F08%2FP297 n14:ED1.100%2F02%2F0123
n3:rokUplatneniVysledku
n13:2012
n3:svazekPeriodika
57
n3:tvurceVysledku
Janečková, Katarína Uhríková, Ivana Doubek, Jaroslav Scheer, Peter Svoboda, Petr Svěráková, Veronika
n3:wos
000301044700003
s:issn
0375-8427
s:numberOfPages
11
n16:organizacniJednotka
16170