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Statements

Subject Item
n2:RIV%2F62157124%3A16170%2F12%3A43871521%21RIV13-MSM-16170___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The aim of our study was to map the situation of swine mycoplasmoses on four farms in the region of Eastern Slovakia. The primary agent of Enzootic pneumonia of swine is Mycoplasma hyopneumoniae. After reviewing the health status of conventional herds and evaluation of clinical symptoms, paired samples of nasal swabs and venous blood samples were collected from 38 pigs with clinical signs of respiratory disease. Nasal swab samples were tested by nested PCR, while blood samples were used to detect antibodies against M. hyopneumoniae by blocking ELISA. The presence of M. hyopneumoniae was confirmed by nested PCR in four pigs (10.5%) and by blocking ELISA in 16 pigs (42.1%) of all four farms. This work presents for the first time comparison of different methods to diagnose M. hyopneumoniae infection on pig farms in Eastern Slovakia. The aim of our study was to map the situation of swine mycoplasmoses on four farms in the region of Eastern Slovakia. The primary agent of Enzootic pneumonia of swine is Mycoplasma hyopneumoniae. After reviewing the health status of conventional herds and evaluation of clinical symptoms, paired samples of nasal swabs and venous blood samples were collected from 38 pigs with clinical signs of respiratory disease. Nasal swab samples were tested by nested PCR, while blood samples were used to detect antibodies against M. hyopneumoniae by blocking ELISA. The presence of M. hyopneumoniae was confirmed by nested PCR in four pigs (10.5%) and by blocking ELISA in 16 pigs (42.1%) of all four farms. This work presents for the first time comparison of different methods to diagnose M. hyopneumoniae infection on pig farms in Eastern Slovakia.
dcterms:title
Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia
skos:prefLabel
Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia
skos:notation
RIV/62157124:16170/12:43871521!RIV13-MSM-16170___
n8:predkladatel
n9:orjk%3A16170
n3:aktivita
n6:I
n3:aktivity
I
n3:cisloPeriodika
4
n3:dodaniDat
n7:2013
n3:domaciTvurceVysledku
n11:9685634 n11:8370346 n11:7283717
n3:druhVysledku
n5:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
130449
n3:idVysledku
RIV/62157124:16170/12:43871521
n3:jazykVysledku
n10:eng
n3:klicovaSlova
antibodies.; Enzootic pneumonia; respiratory diseases; Diagnosis
n3:klicoveSlovo
n4:respiratory%20diseases n4:antibodies. n4:Enzootic%20pneumonia n4:Diagnosis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[0829AA25539D]
n3:nazevZdroje
Acta veterinaria Brno
n3:obor
n19:GJ
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
12
n3:rokUplatneniVysledku
n7:2012
n3:svazekPeriodika
81
n3:tvurceVysledku
Slepecká, Eva Rosenbergová, Kateřina Treml, František Haladová, Eva Zendulková, Dagmar Suli, Judit Ondrejka, Robert Prokeš, Marian Ondrejková, Anna Korytár, Luboš Beníšek, Zdenek Maženský, David
s:issn
0001-7213
s:numberOfPages
5
n18:doi
10.2754/avb201281040327
n16:organizacniJednotka
16170