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Statements

Subject Item
n2:RIV%2F62157124%3A16170%2F12%3A43871260%21RIV13-MSM-16170___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of gamadelta T lymphocytes was found in pyometra samples mainly due to recruitment of gamadelta+/CD8? T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1beta, TNF-alfa, IL-8, IL-17 and IFN-gama) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively. Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of gamadelta T lymphocytes was found in pyometra samples mainly due to recruitment of gamadelta+/CD8? T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1beta, TNF-alfa, IL-8, IL-17 and IFN-gama) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively.
dcterms:title
gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra
skos:prefLabel
gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra
skos:notation
RIV/62157124:16170/12:43871260!RIV13-MSM-16170___
n10:predkladatel
n11:orjk%3A16170
n3:aktivita
n17:O
n3:aktivity
O
n3:cisloPeriodika
3
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n4:4903927 n4:7362366 n4:1949950 n4:4816676
n3:druhVysledku
n16:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
137690
n3:idVysledku
RIV/62157124:16170/12:43871260
n3:jazykVysledku
n13:eng
n3:klicovaSlova
gamadelta Lymphocytes; Chemokines; Flow cytometry; Lymphocyte subsets; Canine
n3:klicoveSlovo
n6:gamadelta%20Lymphocytes n6:Lymphocyte%20subsets n6:Flow%20cytometry n6:Chemokines n6:Canine
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[EB2B43032C2D]
n3:nazevZdroje
Veterinary Journal
n3:obor
n18:FP
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
11
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
194
n3:tvurceVysledku
Matiašovic, Ján Turánek-Knötigová, Pavlína Levá, Lenka Štěpánová, Hana Vitásek, Roman Faldyna, Martin Moore, Peter F. Ondráčková, Petra Oreškovič, Zrinka Vicenová, Monika Bartošková, Alena
s:issn
1090-0233
s:numberOfPages
6
n14:organizacniJednotka
16170