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Statements

Subject Item
n2:RIV%2F62157124%3A16170%2F11%3A43870732%21RIV12-MSM-16170___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p { 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p { 0.05) in experiment 1. The fluid from ovarian cysts } 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO(3), BE, pCO(2) and pO(2) (experiment 2). The indicators were compared between samples 1 and 2. Higher pO(2) as well as pH and lower pCO(2) in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration. The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p { 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p { 0.05) in experiment 1. The fluid from ovarian cysts } 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO(3), BE, pCO(2) and pO(2) (experiment 2). The indicators were compared between samples 1 and 2. Higher pO(2) as well as pH and lower pCO(2) in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration.
dcterms:title
Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle
skos:prefLabel
Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle
skos:notation
RIV/62157124:16170/11:43870732!RIV12-MSM-16170___
n16:predkladatel
n17:orjk%3A16170
n3:aktivita
n4:Z
n3:aktivity
Z(MSM6215712403)
n3:cisloPeriodika
2
n3:dodaniDat
n10:2012
n3:domaciTvurceVysledku
n7:6408419 n7:3799964 n7:8001782 n7:6172202 n7:6714455 n7:1399675
n3:druhVysledku
n9:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
209573
n3:idVysledku
RIV/62157124:16170/11:43870732
n3:jazykVysledku
n12:eng
n3:klicovaSlova
acid-base indicators; cysts; follicles; ovum pick-up; collection of follicular fluid; heifer; Cow
n3:klicoveSlovo
n6:collection%20of%20follicular%20fluid n6:cysts n6:follicles n6:ovum%20pick-up n6:heifer n6:acid-base%20indicators n6:Cow
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[49CC8AD3B9CD]
n3:nazevZdroje
Acta veterinaria Brno
n3:obor
n15:GJ
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n10:2011
n3:svazekPeriodika
80
n3:tvurceVysledku
Doležel, Radovan Lopatářová, Miloslava Pechová, Alena Indrová, Eva Čech, Svatopluk Malá, Jana
n3:wos
000293898400007
n3:zamer
n20:MSM6215712403
s:issn
0001-7213
s:numberOfPages
6
n14:doi
10.2754/avb201180020179
n18:organizacniJednotka
16170