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Statements

Subject Item
n2:RIV%2F62156489%3A_____%2F01%3A21100014%21RIV%2F2002%2FGA0%2F430002%2FN
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Malepszy and Solarek were the first who report somatic embryogenesis from cucumber cell suspension in 1986. The main Iimitation of cucumber embryogenesis is a high occurrence of abnormal embryos combined with a Iow ability and Branchard, 1993). For this work, we report on the induction of somatic embryos in cucumber hypocotyl suspension. The endogenous ABA Ievel in the cells are also discussed. One-week-old plants, derived from the surface sterilized seeds of cucumber (Cucumis sativus L., cv. Nora) grown on a solidified medium (MS, sucrose 5 9 r'), were used. Embryogenic cell suspension cultures from hypocotyl segments of cucumber have been initiated by 7-day exposure to MS medium supplemented with 3% sucrose, 2.2 mg r' 2,4-dichlorophenoxyacetic acid, and 100 mg r' casein hydrolyzate. The suspension cultures were maintained in the same liquid medium with 1.1 mg r1 2,4-D only on a horizontal shaker at 28°C, and 16/8 h photoperiod. Malepszy and Solarek were the first who report somatic embryogenesis from cucumber cell suspension in 1986. The main Iimitation of cucumber embryogenesis is a high occurrence of abnormal embryos combined with a Iow ability and Branchard, 1993). For this work, we report on the induction of somatic embryos in cucumber hypocotyl suspension. The endogenous ABA Ievel in the cells are also discussed. One-week-old plants, derived from the surface sterilized seeds of cucumber (Cucumis sativus L., cv. Nora) grown on a solidified medium (MS, sucrose 5 9 r'), were used. Embryogenic cell suspension cultures from hypocotyl segments of cucumber have been initiated by 7-day exposure to MS medium supplemented with 3% sucrose, 2.2 mg r' 2,4-dichlorophenoxyacetic acid, and 100 mg r' casein hydrolyzate. The suspension cultures were maintained in the same liquid medium with 1.1 mg r1 2,4-D only on a horizontal shaker at 28°C, and 16/8 h photoperiod.
dcterms:title
Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension
skos:prefLabel
Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension
skos:notation
RIV/62156489:_____/01:21100014!RIV/2002/GA0/430002/N
n4:strany
100;100
n4:aktivita
n18:P
n4:aktivity
P(GV206/96/K188)
n4:dodaniDat
n5:2002
n4:domaciTvurceVysledku
n7:1840649 n7:2453290 n7:7298633 n7:3668479
n4:druhVysledku
n17:D
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
696347
n4:idVysledku
RIV/62156489:_____/01:21100014
n4:jazykVysledku
n14:eng
n4:klicovaSlova
cell suspension; Cucumber; in vi¨tro
n4:klicoveSlovo
n16:in%20vi%C2%A8tro n16:Cucumber n16:cell%20suspension
n4:kontrolniKodProRIV
[141D58C565E7]
n4:mistoKonaniAkce
Nitra, Slovak Republic
n4:mistoVydani
Nitra, Slovak Republic
n4:nazevZdroje
Xth International Conference on Plan Embryology
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:pocetUcastnikuAkce
0
n4:pocetZahranicnichUcastnikuAkce
0
n4:projekt
n8:GV206%2F96%2FK188
n4:rokUplatneniVysledku
n5:2001
n4:tvurceVysledku
Andrýsková, Lenka Procházka, Stanislav Smíšková, Alexandra Klemš, Marek
n4:typAkce
n19:EUR
n4:zahajeniAkce
2001-07-01+02:00
s:numberOfPages
1
n10:hasPublisher
Neuveden