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Statements

Subject Item
n2:RIV%2F62156489%3A43510%2F12%3A00193977%21RIV14-MSM-43510___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
European mistletoe (Viscum album), the hemiparasitic shrub, infects a wide range of woody species. It adversely affects the height and diameter growth and it is associated with increased mortality of its hosts. Currently there are no effective control methods against it. Therefore, we started to study a specific hyperparasitic fungus (Phaeobotryosphaeria visci), which can completely destroy European mistletoe by infecting its branches, leaves and berries. An important aspect of the initial phase of mycopesticide candidate is culturing of the organism on artificial or non-synthetic media, followed by the optimization of spore production. We focused to determinate the growth media and light conditions needed for sporulation of P. visci. We also tested the viability and pathogenecy of the spores, because these are important features for further processing and applications. The cultures were grown on seven different media (potato dextrose agar, sugar free potato dextrose agar, cellophane covered potato dextrose agar, oatmeal agar, V8 Juice agar, S medium and SNA medium) under constant dark, constant light (400-750 nm) and 12 h of alternating dark and light illumination. The best primary agar media were oatmeal and sugar free potato dextrose agar under permanent illumination, while constant dark inhibited the conidial production. The viability and virulence of harvested conidia were normal, and the symptoms of the disease appeared 7-14 days after the inoculation on mistletoe leaves. We will continue our experiments studying the effect of near UV (280--400 nm) light. Use of variable photoperiods supplemented with near UV and different media could help us to optimize the spore production and create a fast and cheap mass production technique. European mistletoe (Viscum album), the hemiparasitic shrub, infects a wide range of woody species. It adversely affects the height and diameter growth and it is associated with increased mortality of its hosts. Currently there are no effective control methods against it. Therefore, we started to study a specific hyperparasitic fungus (Phaeobotryosphaeria visci), which can completely destroy European mistletoe by infecting its branches, leaves and berries. An important aspect of the initial phase of mycopesticide candidate is culturing of the organism on artificial or non-synthetic media, followed by the optimization of spore production. We focused to determinate the growth media and light conditions needed for sporulation of P. visci. We also tested the viability and pathogenecy of the spores, because these are important features for further processing and applications. The cultures were grown on seven different media (potato dextrose agar, sugar free potato dextrose agar, cellophane covered potato dextrose agar, oatmeal agar, V8 Juice agar, S medium and SNA medium) under constant dark, constant light (400-750 nm) and 12 h of alternating dark and light illumination. The best primary agar media were oatmeal and sugar free potato dextrose agar under permanent illumination, while constant dark inhibited the conidial production. The viability and virulence of harvested conidia were normal, and the symptoms of the disease appeared 7-14 days after the inoculation on mistletoe leaves. We will continue our experiments studying the effect of near UV (280--400 nm) light. Use of variable photoperiods supplemented with near UV and different media could help us to optimize the spore production and create a fast and cheap mass production technique.
dcterms:title
Optimizing conditions for sporulation of European mistletoe hyperparasitic fungus (Phaeobotryosphaeria visci): effect of light and different media Optimizing conditions for sporulation of European mistletoe hyperparasitic fungus (Phaeobotryosphaeria visci): effect of light and different media
skos:prefLabel
Optimizing conditions for sporulation of European mistletoe hyperparasitic fungus (Phaeobotryosphaeria visci): effect of light and different media Optimizing conditions for sporulation of European mistletoe hyperparasitic fungus (Phaeobotryosphaeria visci): effect of light and different media
skos:notation
RIV/62156489:43510/12:00193977!RIV14-MSM-43510___
n8:predkladatel
n9:orjk%3A43510
n3:aktivita
n4:S
n3:aktivity
S
n3:cisloPeriodika
-
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n14:9952713
n3:druhVysledku
n17:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
157010
n3:idVysledku
RIV/62156489:43510/12:00193977
n3:jazykVysledku
n16:eng
n3:klicovaSlova
oatmeal agar; photoperiod; biological control; bioherbicide; mass production
n3:klicoveSlovo
n10:bioherbicide n10:photoperiod n10:oatmeal%20agar n10:mass%20production n10:biological%20control
n3:kodStatuVydavatele
HU - Maďarsko
n3:kontrolniKodProRIV
[1AE473E8FD8B]
n3:nazevZdroje
Journal of Agricultural Sciences: Acta Agraria Debreceniensis
n3:obor
n18:GK
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n13:2012
n3:svazekPeriodika
50
n3:tvurceVysledku
Baltazár, Tivadar Apró, Melinda Hyvönen, Jaakko Poczai, Péter Varga, Ildikó
s:issn
1588-8363
s:numberOfPages
7
n15:organizacniJednotka
43510