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Statements

Subject Item
n2:RIV%2F62156489%3A43510%2F12%3A00193466%21RIV13-MZE-43510___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were infl uenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a nonlinear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a diff erent eff ectiveness of antioxidants and a diff erent stability of their products with radicals. This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were infl uenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a nonlinear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a diff erent eff ectiveness of antioxidants and a diff erent stability of their products with radicals.
dcterms:title
Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method
skos:prefLabel
Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method
skos:notation
RIV/62156489:43510/12:00193466!RIV13-MZE-43510___
n18:predkladatel
n19:orjk%3A43510
n3:aktivita
n14:P
n3:aktivity
P(QI91B094)
n3:cisloPeriodika
8
n3:dodaniDat
n7:2013
n3:domaciTvurceVysledku
n10:1231154 n10:5942586
n3:druhVysledku
n16:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
133234
n3:idVysledku
RIV/62156489:43510/12:00193466
n3:jazykVysledku
n15:eng
n3:klicovaSlova
antioxidant capacity; photochem; sample dilution; photochemiluminiscence
n3:klicoveSlovo
n5:photochem n5:photochemiluminiscence n5:antioxidant%20capacity n5:sample%20dilution
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[1FECFDCD7F76]
n3:nazevZdroje
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
n3:obor
n13:GC
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n6:QI91B094
n3:rokUplatneniVysledku
n7:2012
n3:svazekPeriodika
60
n3:tvurceVysledku
Balík, Josef Híc, Pavel
s:issn
1211-8516
s:numberOfPages
6
n17:organizacniJednotka
43510