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Statements

Subject Item
n2:RIV%2F62156489%3A43510%2F04%3A00003557%21RIV06-GA0-43510___
rdf:type
skos:Concept n20:Vysledek
dcterms:description
SSR polymorphisms are extensively used as genetic markers. They are generally codominant, highly polymorphic, they have high eficiency in a species with low variability such as peach and can be detected with a simple PCR procedure. Consequently SSR were used for study of the genetic diversity in Prunus genus. The 9 known SSR pair of primers nominated by other autors were applied to 17 Prunus cultivars. SSR analyses were performed with the thermal cycler program by the use of touchdown protocol for annealing The electrophoretic spectrum of the obtained bands on the agarose MetaPhor were similar to the spectrum on polyacrylamide (PAGE). The gel on the Metaphor was visualised by ethidium bromide staining, the polyacrylamide gel by the SYBR Gold. The primers distinguished few a homozygous and heterozygous genotypes in given locus within all analysed cultivars. The given sizes of the SSR products were compared with the resulting product sizes in work of Testolin et al. (2000). These results can be use SSR polymorphisms are extensively used as genetic markers. They are generally codominant, highly polymorphic, they have high eficiency in a species with low variability such as peach and can be detected with a simple PCR procedure. Consequently SSR were used for study of the genetic diversity in Prunus genus. The 9 known SSR pair of primers nominated by other autors were applied to 17 Prunus cultivars. SSR analyses were performed with the thermal cycler program by the use of touchdown protocol for annealing The electrophoretic spectrum of the obtained bands on the agarose MetaPhor were similar to the spectrum on polyacrylamide (PAGE). The gel on the Metaphor was visualised by ethidium bromide staining, the polyacrylamide gel by the SYBR Gold. The primers distinguished few a homozygous and heterozygous genotypes in given locus within all analysed cultivars. The given sizes of the SSR products were compared with the resulting product sizes in work of Testolin et al. (2000). These results can be use SSR polymorphisms are extensively used as genetic markers. They are generally codominant, highly polymorphic, they have high eficiency in a species with low variability such as peach and can be detected with a simple PCR procedure. Consequently SSR were used for study of the genetic diversity in Prunus genus. The 9 known SSR pair of primers nominated by other autors were applied to 17 Prunus cultivars. SSR analyses were performed with the thermal cycler program by the use of touchdown protocol for annealing The electrophoretic spectrum of the obtained bands on the agarose MetaPhor were similar to the spectrum on polyacrylamide (PAGE). The gel on the Metaphor was visualised by ethidium bromide staining, the polyacrylamide gel by the SYBR Gold. The primers distinguished few a homozygous and heterozygous genotypes in given locus within all analysed cultivars. The given sizes of the SSR products were compared with the resulting product sizes in work of Testolin et al. (2000). These results can be use
dcterms:title
SSR analyses within Prunus genus SSR analyses within Prunus genus SSR analyses within Prunus genus
skos:prefLabel
SSR analyses within Prunus genus SSR analyses within Prunus genus SSR analyses within Prunus genus
skos:notation
RIV/62156489:43510/04:00003557!RIV06-GA0-43510___
n3:strany
48;48
n3:aktivita
n15:P
n3:aktivity
P(GP521/03/P133)
n3:dodaniDat
n16:2006
n3:domaciTvurceVysledku
n9:1254928 n9:7910444 n9:8526028 n9:2774771
n3:druhVysledku
n18:D
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
587671
n3:idVysledku
RIV/62156489:43510/04:00003557
n3:jazykVysledku
n4:cze
n3:klicovaSlova
DNA; MetaPhor; PAGE; Prunus; SSR
n3:klicoveSlovo
n6:SSR n6:DNA n6:PAGE n6:Prunus n6:MetaPhor
n3:kontrolniKodProRIV
[632E6ECF365F]
n3:mistoVydani
Brno
n3:nazevZdroje
International Conference on Horticulture Post-graduate (Ph.D.) Study System and Conditions in Europe
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n17:GP521%2F03%2FP133
n3:rokUplatneniVysledku
n16:2004
n3:tvurceVysledku
Baránek, Miroslav Vachůn, Miroslav Raddová, Jana Pidra, Miroslav
s:numberOfPages
1
n10:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n13:isbn
80-7157-801-0
n12:organizacniJednotka
43510