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Statements

Subject Item
n2:RIV%2F62156489%3A43410%2F11%3A00168202%21RIV12-MSM-43410___
rdf:type
skos:Concept n7:Vysledek
dcterms:description
Embryogenic cell masses (ECM) of Abies cephalonica were grown on proliferation media in the presence and absence of fulvic acid (FA), whose molecular composition and conformational rigidity were evaluated by CPMAS-C NMR spectroscopy. To assess the physiological effects of this humic material during proliferation and maturation stages of somatic embryogenesis (SE), proliferation rate, proportion of consecutive developmental stages of pro-embryogenic masses (PEM), cellular ATP and glucose-6-phosphate were evaluated at regular intervals. FA increased the proliferation rate, especially during the early sampling days, and the percentage of PEM in their advanced developmental stage. Cellular ATP and glucose-6-phospahte were increased by FA pre-treatment during the maturation phase. Furthermore, the effects of the anti-auxin p-chlorophenoxyisobutyric acid (PCIB), such as a decrease of growth and the enhancement of PEM III induction, were inverted by FA. Proton pumping ATPase and PPase activities were decreased in microsomes from PCIB-treated ECM, while they increased in the presence of FA. This fulvic matter also induced a delay in somatic embryo formation during the maturation phase. Both the improvement of the PEM proliferation and the reduction of the subsequent maturation process of A. cephalonica are explained by a release from the complex humic structure of low molecular-weight molecules, which may interact with the plant hormonal signaling pathway. These effects appear to be related to the hydrophilic and conformationally labile nature of FA. The structure-activity relationship observed here suggests that the influence of FA on ECM may be attributed to specific bioactive molecules that are preferentially released from the FA loose superstructure. Embryogenic cell masses (ECM) of Abies cephalonica were grown on proliferation media in the presence and absence of fulvic acid (FA), whose molecular composition and conformational rigidity were evaluated by CPMAS-C NMR spectroscopy. To assess the physiological effects of this humic material during proliferation and maturation stages of somatic embryogenesis (SE), proliferation rate, proportion of consecutive developmental stages of pro-embryogenic masses (PEM), cellular ATP and glucose-6-phosphate were evaluated at regular intervals. FA increased the proliferation rate, especially during the early sampling days, and the percentage of PEM in their advanced developmental stage. Cellular ATP and glucose-6-phospahte were increased by FA pre-treatment during the maturation phase. Furthermore, the effects of the anti-auxin p-chlorophenoxyisobutyric acid (PCIB), such as a decrease of growth and the enhancement of PEM III induction, were inverted by FA. Proton pumping ATPase and PPase activities were decreased in microsomes from PCIB-treated ECM, while they increased in the presence of FA. This fulvic matter also induced a delay in somatic embryo formation during the maturation phase. Both the improvement of the PEM proliferation and the reduction of the subsequent maturation process of A. cephalonica are explained by a release from the complex humic structure of low molecular-weight molecules, which may interact with the plant hormonal signaling pathway. These effects appear to be related to the hydrophilic and conformationally labile nature of FA. The structure-activity relationship observed here suggests that the influence of FA on ECM may be attributed to specific bioactive molecules that are preferentially released from the FA loose superstructure.
dcterms:title
Fulvic acid affects proliferation and maturation phases in Abies cephalonica embryogenic cells Fulvic acid affects proliferation and maturation phases in Abies cephalonica embryogenic cells
skos:prefLabel
Fulvic acid affects proliferation and maturation phases in Abies cephalonica embryogenic cells Fulvic acid affects proliferation and maturation phases in Abies cephalonica embryogenic cells
skos:notation
RIV/62156489:43410/11:00168202!RIV12-MSM-43410___
n7:predkladatel
n8:orjk%3A43410
n3:aktivita
n14:S
n3:aktivity
S
n3:cisloPeriodika
11
n3:dodaniDat
n16:2012
n3:domaciTvurceVysledku
n9:8761086
n3:druhVysledku
n17:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
200505
n3:idVysledku
RIV/62156489:43410/11:00168202
n3:jazykVysledku
n12:eng
n3:klicovaSlova
fulvic acids; humic substances; somatic embryogenesis
n3:klicoveSlovo
n5:fulvic%20acids n5:somatic%20embryogenesis n5:humic%20substances
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[33644868E24C]
n3:nazevZdroje
Journal of Plant Physiology
n3:obor
n11:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n16:2011
n3:svazekPeriodika
168
n3:tvurceVysledku
SPACCINI, RICCARDO ELISA, PETRUSSA Krajňáková, Jana VIANELLO, ANGELO ZANCANI, MARCO BERTOLINI, ALBERTO PICCOLO, ALESSANDRO
s:issn
0176-1617
s:numberOfPages
8
n18:organizacniJednotka
43410