This HTML5 document contains 49 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n12http://localhost/temp/predkladatel/
n16http://linked.opendata.cz/resource/domain/vavai/projekt/
n4http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n13http://linked.opendata.cz/ontology/domain/vavai/
n18http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n10http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62156489%3A43410%2F08%3A00116481%21RIV12-GA0-43410___/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n19http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n11http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62156489%3A43410%2F08%3A00116481%21RIV12-GA0-43410___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
The method for supercritical fluid extraction (SFE) of amino acids was adapted and optimized for a dry leaves matrix. The matrix-dependent SFE method uses a mixture of MeOH:H2O:acetonitrile (10:10:1 v/v) as a modifier (0.5 ml in situ) at 70 °C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse columns (4.6 x 75 mm) with aqueous Na2HPO4 buffer of pH 7.8 and ACN:MeOH:water as a mobile phase. In comparison with Soxhlet extraction, SFE gained higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or as a combo according to the matrix, number of samples and levels of ballast compounds. The method for supercritical fluid extraction (SFE) of amino acids was adapted and optimized for a dry leaves matrix. The matrix-dependent SFE method uses a mixture of MeOH:H2O:acetonitrile (10:10:1 v/v) as a modifier (0.5 ml in situ) at 70 °C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse columns (4.6 x 75 mm) with aqueous Na2HPO4 buffer of pH 7.8 and ACN:MeOH:water as a mobile phase. In comparison with Soxhlet extraction, SFE gained higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or as a combo according to the matrix, number of samples and levels of ballast compounds.
dcterms:title
Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection
skos:prefLabel
Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection
skos:notation
RIV/62156489:43410/08:00116481!RIV12-GA0-43410___
n3:aktivita
n19:P n19:Z
n3:aktivity
P(GA526/05/0187), Z(MSM6215648902)
n3:cisloPeriodika
8
n3:dodaniDat
n11:2012
n3:domaciTvurceVysledku
n4:1150936 n4:8683913 n4:6004229 n4:8905177 n4:9830197 n4:9904204
n3:druhVysledku
n17:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
398353
n3:idVysledku
RIV/62156489:43410/08:00116481
n3:jazykVysledku
n14:eng
n3:klicovaSlova
birch leaves; chromatography; amino acids
n3:klicoveSlovo
n7:amino%20acids n7:birch%20leaves n7:chromatography
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[26A2360C5C1E]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n16:GA526%2F05%2F0187
n3:rokUplatneniVysledku
n11:2008
n3:svazekPeriodika
31
n3:tvurceVysledku
Hrdlička, Petr Lojková, Lea Buchta, Ivan Kubáň, Vlastimil Kula, Emanuel Klejdus, Bořivoj
n3:zamer
n18:MSM6215648902
s:issn
1615-9306
s:numberOfPages
11
n12:organizacniJednotka
43410