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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F14%3A00229014%21RIV15-MSM-43210___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Granule-bound starch synthase (GBSS) is a key enzyme in starch biosynthesis pathway. It is responsible for production of amylose, therefore low amylose level has been associated with GBSS deficiency. This enzyme, also known as waxy protein, is encoded by three homologous genes -- Wx-A1, Wx-B1 and Wx-D1 and their dysfunction of any origin causes decrease of amylose content. Starch content and, therefore amylose content, is closely linked with structure of the cereal endosperm. It is known, that mealy endosperm contains more starch than vitreous, which contains more proteins. To identify null alleles of waxy genes in 25 genotypes of wheat with nonstandard colored caryopses, PCR markers were used. To determine the endosperm structure of these genotypes, the non-destructive Light Transflectance Meter (LTm) was used. Null alleles for Wx-B1 locus were observed in 5 tested genotypes, loci Wx-A1 and Wx-D1 were amplified in all genotypes. Percentage of mealy caryopses per sample ranged from 0 to 100 % among tested genotypes. No significant correlation between these two parameters was observed. Granule-bound starch synthase (GBSS) is a key enzyme in starch biosynthesis pathway. It is responsible for production of amylose, therefore low amylose level has been associated with GBSS deficiency. This enzyme, also known as waxy protein, is encoded by three homologous genes -- Wx-A1, Wx-B1 and Wx-D1 and their dysfunction of any origin causes decrease of amylose content. Starch content and, therefore amylose content, is closely linked with structure of the cereal endosperm. It is known, that mealy endosperm contains more starch than vitreous, which contains more proteins. To identify null alleles of waxy genes in 25 genotypes of wheat with nonstandard colored caryopses, PCR markers were used. To determine the endosperm structure of these genotypes, the non-destructive Light Transflectance Meter (LTm) was used. Null alleles for Wx-B1 locus were observed in 5 tested genotypes, loci Wx-A1 and Wx-D1 were amplified in all genotypes. Percentage of mealy caryopses per sample ranged from 0 to 100 % among tested genotypes. No significant correlation between these two parameters was observed.
dcterms:title
Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses
skos:prefLabel
Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses Granule-bound starch synthase and endosperm mealiness correlaton in wheat (Triticum aestivum L.) with nonstandard colored caryopses
skos:notation
RIV/62156489:43210/14:00229014!RIV15-MSM-43210___
n3:aktivita
n16:S
n3:aktivity
S
n3:dodaniDat
n18:2015
n3:domaciTvurceVysledku
n4:4091760 n4:6512348 n4:6124593 n4:8803447 n4:1249940 n4:2488728 n4:2775840
n3:druhVysledku
n10:D
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
18561
n3:idVysledku
RIV/62156489:43210/14:00229014
n3:jazykVysledku
n12:eng
n3:klicovaSlova
mealy endosperm; Triticum aestivum L.; waxy protein; wheat; GBBS
n3:klicoveSlovo
n5:mealy%20endosperm n5:waxy%20protein n5:GBBS n5:wheat n5:Triticum%20aestivum%20L.
n3:kontrolniKodProRIV
[FFBC5587FFEE]
n3:mistoKonaniAkce
Brno, Czech Republic
n3:mistoVydani
Brno, Czech Republic
n3:nazevZdroje
MendelNet 2014 - Proceedings of International PhD Students Conference
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n18:2014
n3:tvurceVysledku
Havel, Ladislav Hřivna, Luděk Mrkvicová, Eva Štiasna, Klára Presinszká, Mária Trojan, Václav Vyhnánek, Tomáš
n3:typAkce
n15:EUR
n3:zahajeniAkce
2014-11-19+01:00
s:numberOfPages
5
n13:hasPublisher
Mendel University in Brno
n8:isbn
978-80-7509-174-1
n19:organizacniJednotka
43210