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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F14%3A00225096%21RIV15-MZE-43210___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://www.researchgate.net/publication/261758775_Electrochemical_and_Spectrometric_Study_of_GFP-AZT_Interaction
dcterms:description
In the last 18 years the highly active antiretroviral therapy (HAART) has been used for the treatment of HIV and consists of the combination of various drugs. The introduction of this type of treatment caused a significant extension of lives of patients and a reduction of side effects. However, the complete recovery of patients with HIV is still impossible. Azidothymidine (AZT) was the first drug used for the treatment of HIV and it is used in a combination with other drugs in HAART. Although the mechanism of effect of this drug is known, the mechanisms of side effects are still not sufficiently clarified. The way how to orient even in these reactions is the use of markers that allow in vivo monitoring of biological processes associated with the drug. As a fluorescent label the green fluorescent protein (GFP) can be used. In this study, we evaluated the link between AZT and GFP by electrochemical methods and also the change of the properties of GFP fluorescence after the binding/intercalating of AZT. We can conclude from the results obtained that the interaction between the drug and GFP takes place, but it does not adversely affect the fluorescent properties of GFP. Therefore, this protein may serve as a suitable fluorescent marker for observation of biological processes. In the last 18 years the highly active antiretroviral therapy (HAART) has been used for the treatment of HIV and consists of the combination of various drugs. The introduction of this type of treatment caused a significant extension of lives of patients and a reduction of side effects. However, the complete recovery of patients with HIV is still impossible. Azidothymidine (AZT) was the first drug used for the treatment of HIV and it is used in a combination with other drugs in HAART. Although the mechanism of effect of this drug is known, the mechanisms of side effects are still not sufficiently clarified. The way how to orient even in these reactions is the use of markers that allow in vivo monitoring of biological processes associated with the drug. As a fluorescent label the green fluorescent protein (GFP) can be used. In this study, we evaluated the link between AZT and GFP by electrochemical methods and also the change of the properties of GFP fluorescence after the binding/intercalating of AZT. We can conclude from the results obtained that the interaction between the drug and GFP takes place, but it does not adversely affect the fluorescent properties of GFP. Therefore, this protein may serve as a suitable fluorescent marker for observation of biological processes.
dcterms:title
Electrochemical and spectrometric study of GFP-AZT interaction Electrochemical and spectrometric study of GFP-AZT interaction
skos:prefLabel
Electrochemical and spectrometric study of GFP-AZT interaction Electrochemical and spectrometric study of GFP-AZT interaction
skos:notation
RIV/62156489:43210/14:00225096!RIV15-MZE-43210___
n4:aktivita
n18:P
n4:aktivity
P(QJ1230066)
n4:cisloPeriodika
7
n4:dodaniDat
n11:2015
n4:domaciTvurceVysledku
n6:3790185 n6:4163435 n6:4995775 n6:2307049 n6:5028361 n6:7957092 n6:8740658
n4:druhVysledku
n17:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
14092
n4:idVysledku
RIV/62156489:43210/14:00225096
n4:jazykVysledku
n5:eng
n4:klicovaSlova
HAART; electrochemical methods; HIV
n4:klicoveSlovo
n9:HIV n9:electrochemical%20methods n9:HAART
n4:kodStatuVydavatele
RS - Srbská republika
n4:kontrolniKodProRIV
[2D282A0DC906]
n4:nazevZdroje
International Journal of Electrochemical Science
n4:obor
n10:CG
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
8
n4:projekt
n8:QJ1230066
n4:rokUplatneniVysledku
n11:2014
n4:svazekPeriodika
9
n4:tvurceVysledku
Kenšová, Renáta Kynický, Jindřich Guráň, Roman Komínková, Markéta Zítka, Ondřej Kizek, René Trnková, Libuše Adam, Vojtěch
n4:wos
336899000007
s:issn
1452-3981
s:numberOfPages
12
n13:organizacniJednotka
43210