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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F14%3A00218609%21RIV15-TA0-43210___
rdf:type
n15:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S1011134414001225
dcterms:description
We analyzed antibacterial effects of several novel phthalocyanines against Escherichia coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 h of exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg/L and four of them were even efficient in the concentration of 0.20 mg/L. Antibacterial activity of neutral and anionic compounds was considerably lower or even negligible. No antibacterial effect was detected when bacteria were exposed without illumination. Binding affinity to bacterial cells was found to represent an important parameter influencing phthalocyanine antibacterial activity that can be modified by total charge of peripheral substituents and by the presence of suitable functional groups inside them. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines (the strongest binders to bacterial membrane) affected cytometric measurements of total cell counts, thus without appropriate pretreatment of the sample before analysis this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. Cytometric measurement of cell membrane integrity appears to be a suitable and even more sensitive parameter than cultivation. We analyzed antibacterial effects of several novel phthalocyanines against Escherichia coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 h of exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg/L and four of them were even efficient in the concentration of 0.20 mg/L. Antibacterial activity of neutral and anionic compounds was considerably lower or even negligible. No antibacterial effect was detected when bacteria were exposed without illumination. Binding affinity to bacterial cells was found to represent an important parameter influencing phthalocyanine antibacterial activity that can be modified by total charge of peripheral substituents and by the presence of suitable functional groups inside them. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines (the strongest binders to bacterial membrane) affected cytometric measurements of total cell counts, thus without appropriate pretreatment of the sample before analysis this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. Cytometric measurement of cell membrane integrity appears to be a suitable and even more sensitive parameter than cultivation.
dcterms:title
Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - Comparison of analytical methods Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - Comparison of analytical methods
skos:prefLabel
Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - Comparison of analytical methods Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - Comparison of analytical methods
skos:notation
RIV/62156489:43210/14:00218609!RIV15-TA0-43210___
n3:aktivita
n5:P n5:I
n3:aktivity
I, P(FR-TI3/196), P(GAP205/10/2280), P(TA01010356)
n3:cisloPeriodika
September 2014
n3:dodaniDat
n6:2015
n3:domaciTvurceVysledku
n8:2001209
n3:druhVysledku
n19:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
15311
n3:idVysledku
RIV/62156489:43210/14:00218609
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Phthalocyanines; Binding affinity; Cultivation techniques; Singlet oxygen; Photosensitizers; Antibacterial effect; Flow cytometry; Cell membrane integrity
n3:klicoveSlovo
n4:Phthalocyanines n4:Flow%20cytometry n4:Binding%20affinity n4:Photosensitizers n4:Singlet%20oxygen n4:Cell%20membrane%20integrity n4:Cultivation%20techniques n4:Antibacterial%20effect
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[F861E9B0F24D]
n3:nazevZdroje
Journal of Photochemistry and Photobiology B: Biology
n3:obor
n20:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:projekt
n13:GAP205%2F10%2F2280 n13:FR-TI3%2F196 n13:TA01010356
n3:rokUplatneniVysledku
n6:2014
n3:svazekPeriodika
138
n3:tvurceVysledku
Černý, Jiří Kalhotka, Libor Maršálek, Blahoslav Jančula, Daniel Zezulka, Štěpán Mikula, Přemysl Kořínková, Radka
n3:wos
340987500027
s:issn
1011-1344
s:numberOfPages
10
n18:doi
10.1016/j.jphotobiol.2014.04.014
n12:organizacniJednotka
43210