This HTML5 document contains 48 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n13http://localhost/temp/predkladatel/
n15http://linked.opendata.cz/resource/domain/vavai/projekt/
n9http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n14http://linked.opendata.cz/resource/domain/vavai/subjekt/
n10http://linked.opendata.cz/ontology/domain/vavai/
n8http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62156489%3A43210%2F13%3A00213595%21RIV14-GA0-43210___/
shttp://schema.org/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n20http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n12http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F13%3A00213595%21RIV14-GA0-43210___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was found to be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported at least 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in saliva by monolithic disc and subsequent offline photometric detection is faster and cheaper method compared to ELISA commercial kit. The total analysis of one sample takes \20 min. In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was found to be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported at least 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in saliva by monolithic disc and subsequent offline photometric detection is faster and cheaper method compared to ELISA commercial kit. The total analysis of one sample takes \20 min.
dcterms:title
Separation of lactoferrin from human saliva using monolithic disc Separation of lactoferrin from human saliva using monolithic disc
skos:prefLabel
Separation of lactoferrin from human saliva using monolithic disc Separation of lactoferrin from human saliva using monolithic disc
skos:notation
RIV/62156489:43210/13:00213595!RIV14-GA0-43210___
n10:predkladatel
n14:orjk%3A43210
n6:aktivita
n7:P
n6:aktivity
P(GAP102/11/1068)
n6:cisloPeriodika
11-12
n6:dodaniDat
n12:2014
n6:domaciTvurceVysledku
n9:2104237 n9:4995775 n9:8082146 n9:8740658 n9:2307049
n6:druhVysledku
n19:J
n6:duvernostUdaju
n11:S
n6:entitaPredkladatele
n8:predkladatel
n6:idSjednocenehoVysledku
104733
n6:idVysledku
RIV/62156489:43210/13:00213595
n6:jazykVysledku
n16:eng
n6:klicovaSlova
lactoferrin; optimalization; separation
n6:klicoveSlovo
n18:optimalization n18:lactoferrin n18:separation
n6:kodStatuVydavatele
DE - Spolková republika Německo
n6:kontrolniKodProRIV
[9C135FBE4383]
n6:nazevZdroje
Chromatographia
n6:obor
n17:CE
n6:pocetDomacichTvurcuVysledku
5
n6:pocetTvurcuVysledku
6
n6:projekt
n15:GAP102%2F11%2F1068
n6:rokUplatneniVysledku
n12:2013
n6:svazekPeriodika
76
n6:tvurceVysledku
Skaličková, Sylvie Vlková, Monika Křížková, Soňa Zítka, Ondřej Kizek, René Adam, Vojtěch
s:issn
0009-5893
s:numberOfPages
9
n20:doi
10.1007/s10337-013-2459-x
n13:organizacniJednotka
43210