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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F13%3A00213473%21RIV14-GA0-43210___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Quantum dots (QDs) belong to a new class of fluorescent agent for biochemical, medicinal or other purposes. However, QDs based on cadmium or other metals can be risky for an organism. As one of the mechanism how to detoxify cadmium-based QDs expression of metallothioneins (MT) can be considered. Due to high affinity of metallothionein to cadmium(II) ions, we attempted to develop an approach for studying of possible interaction with QDs. We prepared QDs with CdTe core and studied the interaction with MT, which we isolated from livers of Cd-administered rabbits. To study the interaction, we used the mixture of both components MT (3.6 uM): CdTe QDs (0, 0.34, 0.68, 1.02, 1.36, 1.7, 2.04 and 2.47 uM). The mixtures were studied by spectrophotometry within the range from 200 to 750 nm with detected maxima at 260 and 505 nm. Same mixtures were also analysed by differential pulse voltammetry Brdicka reaction, which supported data from spectrophotometry. Subsequently, we used fast protein liquid chromatography for purification of protein--quantum dot conjugates. We obtained the different chromatograms for (1) Apo MT, (2) CdTe QDs and (3) MT--QD complex. We also collected the fractions and subsequently analysed them on the content of Cd and MT, which confirmed the formation of CdTe QDs--MT complex. Quantum dots (QDs) belong to a new class of fluorescent agent for biochemical, medicinal or other purposes. However, QDs based on cadmium or other metals can be risky for an organism. As one of the mechanism how to detoxify cadmium-based QDs expression of metallothioneins (MT) can be considered. Due to high affinity of metallothionein to cadmium(II) ions, we attempted to develop an approach for studying of possible interaction with QDs. We prepared QDs with CdTe core and studied the interaction with MT, which we isolated from livers of Cd-administered rabbits. To study the interaction, we used the mixture of both components MT (3.6 uM): CdTe QDs (0, 0.34, 0.68, 1.02, 1.36, 1.7, 2.04 and 2.47 uM). The mixtures were studied by spectrophotometry within the range from 200 to 750 nm with detected maxima at 260 and 505 nm. Same mixtures were also analysed by differential pulse voltammetry Brdicka reaction, which supported data from spectrophotometry. Subsequently, we used fast protein liquid chromatography for purification of protein--quantum dot conjugates. We obtained the different chromatograms for (1) Apo MT, (2) CdTe QDs and (3) MT--QD complex. We also collected the fractions and subsequently analysed them on the content of Cd and MT, which confirmed the formation of CdTe QDs--MT complex.
dcterms:title
Study of interaction between metallothionein and CdTe quantum dots Study of interaction between metallothionein and CdTe quantum dots
skos:prefLabel
Study of interaction between metallothionein and CdTe quantum dots Study of interaction between metallothionein and CdTe quantum dots
skos:notation
RIV/62156489:43210/13:00213473!RIV14-GA0-43210___
n8:predkladatel
n9:orjk%3A43210
n4:aktivita
n6:P
n4:aktivity
P(GA102/08/1546), P(IAA600110902)
n4:cisloPeriodika
7-8
n4:dodaniDat
n16:2014
n4:domaciTvurceVysledku
n13:8082146 n13:5163021 n13:5530237 n13:8594716 n13:2307049 n13:8740658 n13:4995775 n13:2104237 n13:8541698 n13:2032279
n4:druhVysledku
n19:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
108719
n4:idVysledku
RIV/62156489:43210/13:00213473
n4:jazykVysledku
n10:eng
n4:klicovaSlova
metallothionein; interaction; quantum dots
n4:klicoveSlovo
n5:interaction n5:quantum%20dots n5:metallothionein
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[54889AB38F02]
n4:nazevZdroje
Chromatographia
n4:obor
n12:CB
n4:pocetDomacichTvurcuVysledku
10
n4:pocetTvurcuVysledku
12
n4:projekt
n11:IAA600110902 n11:GA102%2F08%2F1546
n4:rokUplatneniVysledku
n16:2013
n4:svazekPeriodika
76
n4:tvurceVysledku
Janů, Libor Zidek, Václav Ryvolová, Markéta Zidkova, Jarmila Hynek, David Kizek, René Skaličková, Sylvie Křížková, Soňa Sochor, Jiří Nejdl, Lukáš Zítka, Ondřej Adam, Vojtěch
n4:wos
316819600007
s:issn
0009-5893
s:numberOfPages
9
n20:doi
10.1007/s10337-013-2418-6
n15:organizacniJednotka
43210