This HTML5 document contains 39 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n18http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62156489%3A43210%2F13%3A00212044%21RIV14-MSM-43210___/
n9http://localhost/temp/predkladatel/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/resource/domain/vavai/subjekt/
n14http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n10http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F13%3A00212044%21RIV14-MSM-43210___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10-12 mol/L for sarin, 6.31 x 10-12 mol /L for soman, 6.17 x 10-11 mol/L for tabun, and 2.19 x 10-11 mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples. The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10-12 mol/L for sarin, 6.31 x 10-12 mol /L for soman, 6.17 x 10-11 mol/L for tabun, and 2.19 x 10-11 mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples.
dcterms:title
Acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents Acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents
skos:prefLabel
Acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents Acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents
skos:notation
RIV/62156489:43210/13:00212044!RIV14-MSM-43210___
n14:predkladatel
n15:orjk%3A43210
n3:aktivita
n16:O
n3:aktivity
O
n3:cisloPeriodika
9
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n7:4995775 n7:2307049
n3:druhVysledku
n13:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
59333
n3:idVysledku
RIV/62156489:43210/13:00212044
n3:jazykVysledku
n4:eng
n3:klicovaSlova
neurotransmitter; acetylcholinesterase
n3:klicoveSlovo
n10:neurotransmitter n10:acetylcholinesterase
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[56E01891A667]
n3:nazevZdroje
Sensors
n3:obor
n17:CG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
13
n3:tvurceVysledku
Kizek, René Pohanka, Miroslav Adam, Vojtěch
s:issn
1424-8220
s:numberOfPages
9
n9:organizacniJednotka
43210