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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F13%3A00210092%21RIV14-MSM-43210___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The aim of this study was to evaluate the effect of age on sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs. We evaluated 90 semen samples. The dogs were divided into groups according to the age (A: 1.5--2 years, B: 2--5 years, C: 5--6.5 years). Semen samples were collected by manual manipulation into the glass beaker. Immediately after collection of macroscopic examination was made for all samples, which included find out volume of ejaculate, sperm activity, concentration and sperm cell viability. Volume was measured using the graduated cylinder. Concentration was evaluated by haematocytometry method using Bürker chamber and activity by subjective method according to the percentage of motile sperm in the native ejaculate. We evaluated the percentage of sperm with progressive direct movement after the head. Viability eosin-nigrosin stain method was performed for evaluation. In this case, we evaluated the total number of alive and dead sperms. Monitored characteristics were expressed in weighted average and standard error. Based on the results we can state that, in case of monitoring factors, the age of dogs had the significant influence. In case of activity, as well as viability, statistically highly significant differences (P < 0.01) were observed between group of youngest dogs (A: 1.5--2 years) and oldest dogs (C: 5--6.5 years). Statistically significant difference (P < 0.05) was proved between dogs from group B (2--5 years) and dogs from group C (5--6.5 years). In conclusion, the negative correlation of age, in case of activity (r = -0.44; P < 0.001) and even viability (r = -0.33; P < 0.01), was demonstrated. With increasing age, the values of both factors were reduced. In case of total number of spermatozoa, this phenomenon was not observed (r = 0.01; P > 0.05). The aim of this study was to evaluate the effect of age on sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs. We evaluated 90 semen samples. The dogs were divided into groups according to the age (A: 1.5--2 years, B: 2--5 years, C: 5--6.5 years). Semen samples were collected by manual manipulation into the glass beaker. Immediately after collection of macroscopic examination was made for all samples, which included find out volume of ejaculate, sperm activity, concentration and sperm cell viability. Volume was measured using the graduated cylinder. Concentration was evaluated by haematocytometry method using Bürker chamber and activity by subjective method according to the percentage of motile sperm in the native ejaculate. We evaluated the percentage of sperm with progressive direct movement after the head. Viability eosin-nigrosin stain method was performed for evaluation. In this case, we evaluated the total number of alive and dead sperms. Monitored characteristics were expressed in weighted average and standard error. Based on the results we can state that, in case of monitoring factors, the age of dogs had the significant influence. In case of activity, as well as viability, statistically highly significant differences (P < 0.01) were observed between group of youngest dogs (A: 1.5--2 years) and oldest dogs (C: 5--6.5 years). Statistically significant difference (P < 0.05) was proved between dogs from group B (2--5 years) and dogs from group C (5--6.5 years). In conclusion, the negative correlation of age, in case of activity (r = -0.44; P < 0.001) and even viability (r = -0.33; P < 0.01), was demonstrated. With increasing age, the values of both factors were reduced. In case of total number of spermatozoa, this phenomenon was not observed (r = 0.01; P > 0.05).
dcterms:title
Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs
skos:prefLabel
Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs
skos:notation
RIV/62156489:43210/13:00210092!RIV14-MSM-43210___
n8:predkladatel
n11:orjk%3A43210
n3:aktivita
n13:S
n3:aktivity
S
n3:dodaniDat
n18:2014
n3:domaciTvurceVysledku
n6:3462048 n6:8517738 n6:6400728 n6:4651545
n3:druhVysledku
n20:D
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
71348
n3:idVysledku
RIV/62156489:43210/13:00210092
n3:jazykVysledku
n15:eng
n3:klicovaSlova
dog ejaculate; age; sperm cell viability; total number of spermatozoa; sperm activity
n3:klicoveSlovo
n9:dog%20ejaculate n9:age n9:sperm%20cell%20viability n9:total%20number%20of%20spermatozoa n9:sperm%20activity
n3:kontrolniKodProRIV
[B08CCA701820]
n3:mistoKonaniAkce
Mendelova univerzita v Brně
n3:mistoVydani
Faculty of Agronomy
n3:nazevZdroje
MendelNet 2013 - Proceedings of International PhD Students Conference
n3:obor
n17:EG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n18:2013
n3:tvurceVysledku
Paldusová, Michaela Hošek, Martin Filipčík, Radek Máchal, Ladislav
n3:typAkce
n16:WRD
n3:zahajeniAkce
2013-11-20+01:00
s:numberOfPages
5
n14:hasPublisher
Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
n21:isbn
978-80-7375-908-7
n10:organizacniJednotka
43210