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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F13%3A00209872%21RIV14-MSM-43210___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene expression 25 days post anthesis (dpa) and the lowest 15 and 35 dpa. In Tschermaks Blaukörniger Sommerweizen was observed the lowest gene expression 40 dpa and the highest 10 dpa, while in genotype UC66049 was DFR gene expression almost the lowest 10 dpa and 40 dpa reached the lowest values. The highest DFR gene expression in Abyssinskaya arraseita was observed between 15 and 20 dpa and then rapidly decreases to its lowest level 25 dpa. Genotype ANK28B shows decreasing gene expression during maturation, the highest value was observed 10 dpa and the lowest 35 dpa, 40 dpa gene expression slightly increased again. Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene expression 25 days post anthesis (dpa) and the lowest 15 and 35 dpa. In Tschermaks Blaukörniger Sommerweizen was observed the lowest gene expression 40 dpa and the highest 10 dpa, while in genotype UC66049 was DFR gene expression almost the lowest 10 dpa and 40 dpa reached the lowest values. The highest DFR gene expression in Abyssinskaya arraseita was observed between 15 and 20 dpa and then rapidly decreases to its lowest level 25 dpa. Genotype ANK28B shows decreasing gene expression during maturation, the highest value was observed 10 dpa and the lowest 35 dpa, 40 dpa gene expression slightly increased again.
dcterms:title
Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
skos:prefLabel
Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
skos:notation
RIV/62156489:43210/13:00209872!RIV14-MSM-43210___
n8:predkladatel
n20:orjk%3A43210
n3:aktivita
n14:S
n3:aktivity
S
n3:dodaniDat
n6:2014
n3:domaciTvurceVysledku
n4:4144406 n4:1249940 n4:2488728 n4:6512348 n4:4091760
n3:druhVysledku
n11:D
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n21:predkladatel
n3:idSjednocenehoVysledku
76427
n3:idVysledku
RIV/62156489:43210/13:00209872
n3:jazykVysledku
n5:eng
n3:klicovaSlova
wheat; dihydroflavonol reductase; genomic analysis
n3:klicoveSlovo
n9:genomic%20analysis n9:wheat n9:dihydroflavonol%20reductase
n3:kontrolniKodProRIV
[A793D9E447BA]
n3:mistoKonaniAkce
Mendelova univerzita v Brně
n3:mistoVydani
Faculty of Agronomy
n3:nazevZdroje
MendelNet 2013 - Proceedings of International PhD Students Conference
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n6:2013
n3:tvurceVysledku
Štiasna, Klára Sekanina, Petr Trojan, Václav Bartoš, Jan Vyhnánek, Tomáš Havel, Ladislav
n3:typAkce
n15:WRD
n3:zahajeniAkce
2013-11-20+01:00
s:numberOfPages
5
n17:hasPublisher
Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
n16:isbn
978-80-7375-908-7
n7:organizacniJednotka
43210