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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F13%3A00199403%21RIV14-GA0-43210___
rdf:type
skos:Concept n11:Vysledek
rdfs:seeAlso
http://www.jmbfs.org/wp-content/uploads/2013/04/jmbfs_0369_ondrouskova.pdf
dcterms:description
For the detection of genetic variability ten genotypes of winter triticale (x Triticosecale Wittmack, 2n = 6x = 42; BBAARR) were selected: nine varieties and one breeding line with good bread-making quality KM 4-09 with the chromosome translocation 1R.1D 5+10-2. 25 microsatellites markers located in the genome A, B, D and R were chosen for analysis. Eighty-four alleles were detected with an average of 3.36 alleles per locus were detected. For each microsatellite statistical values were calculated diversity index (DI), probabilities of identity (PI) and polymorphic information content (PIC) were calculated and averages statistical values are: DI 0.55, PI 0.27 and 0.5 PIC. Overall dendrogram based on the UPGMA method (Jaccards similarity coefficient) significantly distinguished two groups of genotypes and these groups were divided into sub-clusters. A set of 5 SSR markers (Xwms0752, Xbarc128, Xrems1237, Xwms0861 and Xbarc170) which have the calculated PIC value higher than 0.68 that are sufficient for the identification of the analyzed genotypes was described. For the detection of genetic variability ten genotypes of winter triticale (x Triticosecale Wittmack, 2n = 6x = 42; BBAARR) were selected: nine varieties and one breeding line with good bread-making quality KM 4-09 with the chromosome translocation 1R.1D 5+10-2. 25 microsatellites markers located in the genome A, B, D and R were chosen for analysis. Eighty-four alleles were detected with an average of 3.36 alleles per locus were detected. For each microsatellite statistical values were calculated diversity index (DI), probabilities of identity (PI) and polymorphic information content (PIC) were calculated and averages statistical values are: DI 0.55, PI 0.27 and 0.5 PIC. Overall dendrogram based on the UPGMA method (Jaccards similarity coefficient) significantly distinguished two groups of genotypes and these groups were divided into sub-clusters. A set of 5 SSR markers (Xwms0752, Xbarc128, Xrems1237, Xwms0861 and Xbarc170) which have the calculated PIC value higher than 0.68 that are sufficient for the identification of the analyzed genotypes was described.
dcterms:title
Study of genetic variability of triticale varieties by SSR markers Study of genetic variability of triticale varieties by SSR markers
skos:prefLabel
Study of genetic variability of triticale varieties by SSR markers Study of genetic variability of triticale varieties by SSR markers
skos:notation
RIV/62156489:43210/13:00199403!RIV14-GA0-43210___
n11:predkladatel
n15:orjk%3A43210
n3:aktivita
n5:P
n3:aktivity
P(GD204/09/H002)
n3:cisloPeriodika
5
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n4:6512348 n4:1835203
n3:druhVysledku
n10:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
108705
n3:idVysledku
RIV/62156489:43210/13:00199403
n3:jazykVysledku
n18:eng
n3:klicovaSlova
SSR; microsatellites; xTriticosecale Wittmack; triticale; genetic variability
n3:klicoveSlovo
n7:triticale n7:SSR n7:genetic%20variability n7:microsatellites n7:xTriticosecale%20Wittmack
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[1116771D51BB]
n3:nazevZdroje
The Journal of Microbiology, Biotechnology and Food Sciences
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n19:GD204%2F09%2FH002
n3:rokUplatneniVysledku
n13:2013
n3:svazekPeriodika
2
n3:tvurceVysledku
Vyhnánek, Tomáš Ondroušková, Jana
s:issn
1338-5178
s:numberOfPages
3
n20:organizacniJednotka
43210