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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F12%3A00198107%21RIV13-GA0-43210___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated in roots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 uM CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indicate protective role of metallothionein as well as its role in cadmium accumulation in roots of transgenic plants. Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated in roots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 uM CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indicate protective role of metallothionein as well as its role in cadmium accumulation in roots of transgenic plants.
dcterms:title
Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry
skos:prefLabel
Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry
skos:notation
RIV/62156489:43210/12:00198107!RIV13-GA0-43210___
n8:predkladatel
n17:orjk%3A43210
n4:aktivita
n16:P
n4:aktivity
P(ED1.1.00/02.0068), P(GA102/08/1546), P(GA522/07/0692)
n4:cisloPeriodika
2
n4:dodaniDat
n13:2013
n4:domaciTvurceVysledku
n6:4489705 n6:2104237 Shestivska, Violetta n6:9600426 n6:4995775 n6:2645572 n6:8740658 n6:1249940 n6:2307049 n6:8541698 n6:6218482
n4:druhVysledku
n14:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
120865
n4:idVysledku
RIV/62156489:43210/12:00198107
n4:jazykVysledku
n19:eng
n4:klicovaSlova
yeast gene; phytoremediation; metallothionein
n4:klicoveSlovo
n7:phytoremediation n7:metallothionein n7:yeast%20gene
n4:kodStatuVydavatele
RS - Srbská republika
n4:kontrolniKodProRIV
[BE4FE481EF48]
n4:nazevZdroje
International Journal of Electrochemical Science
n4:obor
n18:CG
n4:pocetDomacichTvurcuVysledku
11
n4:pocetTvurcuVysledku
13
n4:projekt
n5:GA102%2F08%2F1546 n5:GA522%2F07%2F0692 n5:ED1.1.00%2F02.0068
n4:rokUplatneniVysledku
n13:2012
n4:svazekPeriodika
7
n4:tvurceVysledku
Babula, Petr Zehnálek, Josef Macek, Tomáš Hubálek, Jaromír Kizek, René Shestivska, Violetta Mackova, Martina Hynek, David Zítka, Ondřej Kryštofová, Olga Adam, Vojtěch Havel, Ladislav Křížková, Soňa
s:issn
1452-3981
s:numberOfPages
22
n15:organizacniJednotka
43210