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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F12%3A00193598%21RIV13-GA0-43210___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Transmissible spongiform encephalopathies are a group of fatal neurodegenerative diseases with long incubation time. This group includes Creutzfeld-Jakob disease, kuru, scrapie, chronic wasting disease, and bovine spongiform encephalopathy. Sensitive and specific detection of abnormal prion protein as %22a source agent%22 of the above-mentioned diseases in blood could provide a diagnostic test or a screening assay for animal and human prion protein diseases diagnostics. Therefore, diagnostic tests for prion protein diseases represent unique challenge requiring development of novel assays exploiting properties of prion protein complex. Presently, diagnostic methods such as protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, fluorescence correlation spectroscopy, and/or flow microbead immunoassay are used for abnormal prion protein (PrPSc) detection. On the other hand, using of CE for PrPSc detection in body fluids is an attractive alternative; it has been already applied for the blood samples of infected sheep, elk, chimpanzee, as well as humans. In this review, assays for prion protein detection are summarized with special attention to capillary electromigration based techniques, such as CE, CIEF, and/or CGE. The potential of the miniaturized and integrated lab-on-chip devices is highlighted, emphasizing recent advances of this field in the proteomic analysis. Transmissible spongiform encephalopathies are a group of fatal neurodegenerative diseases with long incubation time. This group includes Creutzfeld-Jakob disease, kuru, scrapie, chronic wasting disease, and bovine spongiform encephalopathy. Sensitive and specific detection of abnormal prion protein as %22a source agent%22 of the above-mentioned diseases in blood could provide a diagnostic test or a screening assay for animal and human prion protein diseases diagnostics. Therefore, diagnostic tests for prion protein diseases represent unique challenge requiring development of novel assays exploiting properties of prion protein complex. Presently, diagnostic methods such as protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, fluorescence correlation spectroscopy, and/or flow microbead immunoassay are used for abnormal prion protein (PrPSc) detection. On the other hand, using of CE for PrPSc detection in body fluids is an attractive alternative; it has been already applied for the blood samples of infected sheep, elk, chimpanzee, as well as humans. In this review, assays for prion protein detection are summarized with special attention to capillary electromigration based techniques, such as CE, CIEF, and/or CGE. The potential of the miniaturized and integrated lab-on-chip devices is highlighted, emphasizing recent advances of this field in the proteomic analysis.
dcterms:title
Capillary electromigration based techniques in diagnostics of prion protein caused diseases Capillary electromigration based techniques in diagnostics of prion protein caused diseases
skos:prefLabel
Capillary electromigration based techniques in diagnostics of prion protein caused diseases Capillary electromigration based techniques in diagnostics of prion protein caused diseases
skos:notation
RIV/62156489:43210/12:00193598!RIV13-GA0-43210___
n6:predkladatel
n13:orjk%3A43210
n3:aktivita
n19:P
n3:aktivity
P(GAP102/11/1068)
n3:cisloPeriodika
24
n3:dodaniDat
n11:2013
n3:domaciTvurceVysledku
n7:2032279 n7:7333323 n7:4995775 n7:2307049
n3:druhVysledku
n20:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
125944
n3:idVysledku
RIV/62156489:43210/12:00193598
n3:jazykVysledku
n8:eng
n3:klicovaSlova
prion protein; spongiform encephalopathies; imunoanalysis
n3:klicoveSlovo
n5:prion%20protein n5:spongiform%20encephalopathies n5:imunoanalysis
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[0BEB6BA4DEC0]
n3:nazevZdroje
Electrophoresis
n3:obor
n18:CB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n10:GAP102%2F11%2F1068
n3:rokUplatneniVysledku
n11:2012
n3:svazekPeriodika
33
n3:tvurceVysledku
Kizek, René Adam, Vojtěch Šobrová, Pavlína Ryvolová, Markéta
n3:wos
312548900007
s:issn
0173-0835
s:numberOfPages
9
n14:doi
10.1002/elps.201200208
n9:organizacniJednotka
43210