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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F12%3A00189330%21RIV13-GA0-43210___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of ul of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 ug Fe ions/ml) and group of animals suffering from melanoma progression (3.5 ug Fe ions/ml) were determined. Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of ul of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 ug Fe ions/ml) and group of animals suffering from melanoma progression (3.5 ug Fe ions/ml) were determined.
dcterms:title
Automated electrochemical detection of iron ions in erythrocytes from MeLiM minipigs suffering from melanoma Automated electrochemical detection of iron ions in erythrocytes from MeLiM minipigs suffering from melanoma
skos:prefLabel
Automated electrochemical detection of iron ions in erythrocytes from MeLiM minipigs suffering from melanoma Automated electrochemical detection of iron ions in erythrocytes from MeLiM minipigs suffering from melanoma
skos:notation
RIV/62156489:43210/12:00189330!RIV13-GA0-43210___
n11:predkladatel
n16:orjk%3A43210
n3:aktivita
n6:P n6:Z
n3:aktivity
P(ED1.1.00/02.0068), P(GA102/08/1546), Z(AV0Z50450515)
n3:cisloPeriodika
7
n3:dodaniDat
n15:2013
n3:domaciTvurceVysledku
n4:9100318 n4:4995775 n4:1774395 n4:4489705 n4:8184208 n4:8594716 n4:2307049 n4:8541698 n4:9584366
n3:druhVysledku
n8:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
124177
n3:idVysledku
RIV/62156489:43210/12:00189330
n3:jazykVysledku
n9:eng
n3:klicovaSlova
melanoma; electrochemical detection; erythrocytes
n3:klicoveSlovo
n10:erythrocytes n10:electrochemical%20detection n10:melanoma
n3:kodStatuVydavatele
RS - Srbská republika
n3:kontrolniKodProRIV
[90453786C5B3]
n3:nazevZdroje
International Journal of Electrochemical Science
n3:obor
n12:CB
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
12
n3:projekt
n13:GA102%2F08%2F1546 n13:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n15:2012
n3:svazekPeriodika
7
n3:tvurceVysledku
Sochor, Jiří Barath, Petr Kizek, René Cernei, Natalia Horák, V. Majzlík, Petr Hubálek, Jaromír Kremplová, Monika Hynek, David Vrba, R. Krejčová, Ludmila Adam, Vojtěch
n3:wos
306399700012
n3:zamer
n20:AV0Z50450515
s:issn
1452-3981
s:numberOfPages
17
n7:organizacniJednotka
43210