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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F12%3A00188697%21RIV13-GA0-43210___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize beta-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin-O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows an almost complete reversion in the root elongation assay. This is the first evidence from intact plants that the vacuole is the storage organelle for CK O-glucosides and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1 as a robust molecular tool that exploits the reversibility of O-glucosylation and enables delicate manipulations of active CK content at the cellular level. Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize beta-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin-O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows an almost complete reversion in the root elongation assay. This is the first evidence from intact plants that the vacuole is the storage organelle for CK O-glucosides and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1 as a robust molecular tool that exploits the reversibility of O-glucosylation and enables delicate manipulations of active CK content at the cellular level.
dcterms:title
Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole
skos:prefLabel
Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole
skos:notation
RIV/62156489:43210/12:00188697!RIV13-GA0-43210___
n10:predkladatel
n13:orjk%3A43210
n3:aktivita
n6:S n6:P n6:Z
n3:aktivity
P(1M06030), P(GP204/09/P289), P(IAA600380507), P(LC06034), S, Z(AV0Z50040507), Z(AV0Z50040702), Z(AV0Z50380511)
n3:cisloPeriodika
1
n3:dodaniDat
n16:2013
n3:domaciTvurceVysledku
n12:9462791 n12:9071105 n12:6876714
n3:druhVysledku
n11:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
165344
n3:idVysledku
RIV/62156489:43210/12:00188697
n3:jazykVysledku
n21:eng
n3:klicovaSlova
Tobacco; Storage organelle; Cytokinin metabolism; Nicotiana tabacum; Vacuole; beta-Glucosidase; Sub-cellular compartmentation; Zeatin-O-glucoside
n3:klicoveSlovo
n4:Tobacco n4:Cytokinin%20metabolism n4:beta-Glucosidase n4:Storage%20organelle n4:Sub-cellular%20compartmentation n4:Nicotiana%20tabacum n4:Vacuole n4:Zeatin-O-glucoside
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[5E792C2C08D4]
n3:nazevZdroje
Phytochemistry
n3:obor
n5:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:projekt
n15:LC06034 n15:1M06030 n15:IAA600380507 n15:GP204%2F09%2FP289
n3:rokUplatneniVysledku
n16:2012
n3:svazekPeriodika
79
n3:tvurceVysledku
Malbeck, Jiří Brzobohatý, Břetislav Dubová, Jaroslava Palme, Klaus Benková, Eva Kiran, Nagavalli Subbanna Reková, Alena
n3:wos
307031800006
n3:zamer
n8:AV0Z50380511 n8:AV0Z50040702 n8:AV0Z50040507
s:issn
0031-9422
s:numberOfPages
12
n9:doi
10.1016/j.phytochem.2012.03.012
n18:organizacniJednotka
43210