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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F11%3A00183669%21RIV12-GA0-43210___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
The oxidative stress and its influence on live organism have been studied for several decades. Both animals and plants has various mechanisms to minimize of influence of oxidative stress nevertheless the mechanisms belongs to plants are little more advanced especially if we considering heavy metal ion affects. Simply said plant has limited options in case of escape from polluted areas in comprise with animals. One of most developed mechanism which plants have developed to be resistant against heavy metal ion affecting is producing of peptides called phytochelatines. The synthesis of phytochelatins is catalyzed by g-Glu-Cys dipeptidyl transpeptidase phytochelatin synthase (PCS). Aim of this work was to suggest new tool (high performance liquid chromatography coupled with electrochemical detector-HPLC-ED) for determination of the phytochelatin synthase activity. The optimized tool was subsequently used for study PCS activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, we conducted an experiment on BY-2 cells, which were exposed to different concentrations of 0, 5, 10, 25, 50 and 100 uM Cd(NO3)2 for three days. Cells were harvested and homogenised in phosphate buffer (0.2 M, pH 7 with 1M TCEP). Next step for detection an activity of PCS was an adition of reduced glutathione in various concentrations from 0.05 to 5 mM and cadmium(II) ions 50 uM as our optimized protocol to the cell suspension supernatant. These mixtures were incubated at 35 °C for 30 min and analysed using HPLC-ED in optimized conditions mobile phase A:80mM and B: 100% methanol under the best detection potential 900mV. It clearly follows from the results obtained that concentration of PC2 and thus PCS activity enhanced markedly with increasing concentration of cadmium(II) ions. The lowest concentration of the toxic metal ions caused almost three times enhancing PCS activity compared to control samples. According obtained results we were The oxidative stress and its influence on live organism have been studied for several decades. Both animals and plants has various mechanisms to minimize of influence of oxidative stress nevertheless the mechanisms belongs to plants are little more advanced especially if we considering heavy metal ion affects. Simply said plant has limited options in case of escape from polluted areas in comprise with animals. One of most developed mechanism which plants have developed to be resistant against heavy metal ion affecting is producing of peptides called phytochelatines. The synthesis of phytochelatins is catalyzed by g-Glu-Cys dipeptidyl transpeptidase phytochelatin synthase (PCS). Aim of this work was to suggest new tool (high performance liquid chromatography coupled with electrochemical detector-HPLC-ED) for determination of the phytochelatin synthase activity. The optimized tool was subsequently used for study PCS activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, we conducted an experiment on BY-2 cells, which were exposed to different concentrations of 0, 5, 10, 25, 50 and 100 uM Cd(NO3)2 for three days. Cells were harvested and homogenised in phosphate buffer (0.2 M, pH 7 with 1M TCEP). Next step for detection an activity of PCS was an adition of reduced glutathione in various concentrations from 0.05 to 5 mM and cadmium(II) ions 50 uM as our optimized protocol to the cell suspension supernatant. These mixtures were incubated at 35 °C for 30 min and analysed using HPLC-ED in optimized conditions mobile phase A:80mM and B: 100% methanol under the best detection potential 900mV. It clearly follows from the results obtained that concentration of PC2 and thus PCS activity enhanced markedly with increasing concentration of cadmium(II) ions. The lowest concentration of the toxic metal ions caused almost three times enhancing PCS activity compared to control samples. According obtained results we were
dcterms:title
Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions
skos:prefLabel
Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions
skos:notation
RIV/62156489:43210/11:00183669!RIV12-GA0-43210___
n12:predkladatel
n14:orjk%3A43210
n3:aktivita
n4:P
n3:aktivity
P(GA522/07/0692)
n3:cisloPeriodika
1
n3:dodaniDat
n11:2012
n3:domaciTvurceVysledku
n5:2645572 n5:4995775 n5:8740658 n5:2307049 n5:6218482
n3:druhVysledku
n18:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
213590
n3:idVysledku
RIV/62156489:43210/11:00183669
n3:jazykVysledku
n15:eng
n3:klicovaSlova
mechanism; cadmium ions; phytochelatin synthas
n3:klicoveSlovo
n8:mechanism n8:cadmium%20ions n8:phytochelatin%20synthas
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[9A6FCC52F5CD]
n3:nazevZdroje
Interdisciplinary Toxicology
n3:obor
n17:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n13:GA522%2F07%2F0692
n3:rokUplatneniVysledku
n11:2011
n3:svazekPeriodika
4
n3:tvurceVysledku
Zítka, Ondřej Zehnálek, Josef Kizek, René Kryštofová, Olga Adam, Vojtěch
s:issn
1337-6853
s:numberOfPages
2
n16:organizacniJednotka
43210