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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F11%3A00180359%21RIV12-MSM-43210___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Heavy metals are classified as priority pollutants monitored in environments. Heavy metals are not biodegradable, having ability to accumulate in organisms. Cadmium is one of the most toxic heavy metal ions in the environment due to its high mobility and severe toxicity to the organisms. Zinc is an essential micronutrient for plants but can be highly toxic when present at excessive concentration. We aimed at investigation of detoxification mechanisms of maize plants (Zea mays L.) treated with the following combinations of zinc(II) and cadmium(II) ions 0 mM Zn2+ + 0 mM Cd2+; 100 mM Zn2+ + 0 mM Cd2+; 0 mM Zn2+ + 100 mM Cd2+; 10 mM Zn2+ + 100 mM Cd2+; 50 mM Zn2+ + 100 mM Cd2+; 75 mM Zn2+ + 100 mM Cd2+ and 100 mM Zn2+ + 100 mM Cd2+ for 10 days. In addition to growth parameters, we electrochemically determined the metal content in plants. To determine the antioxidant activity, we chose three methods FRAP, DPPH and ABTS, which differ in the spectrum of molecules with possible antioxidant activity. Pearson's correlation coefficient was found by comparison of FRAP and ABTS methods - rP = 0.7967 (Bradford) and 0.7957 (Pyrogallol Red). Pearson's correlation coefficients indicates mutual correlation of both DPPH and ABTS methods - rP = 0.8292 (Bradford) and 0.8737 (Pyrogallol Red). The highest correlation of Pearson's coefficients was found in comparison between DPPH and FRAP methods (0.9407 -- Bradford and 0.9211 Pyrogallol Red), suggesting a very high dependence of the two methods. Thus, our attention was focused on the determination of activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). We were also focused on the monitoring of the GST activity. In addition, we monitored its correlation with total content of thiol compounds (-SH) measured spectrometrically using Ellman's method. Content of thiol compounds, such as reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatins (PC2, PC5) was determined by HPLC--ED. The hi Heavy metals are classified as priority pollutants monitored in environments. Heavy metals are not biodegradable, having ability to accumulate in organisms. Cadmium is one of the most toxic heavy metal ions in the environment due to its high mobility and severe toxicity to the organisms. Zinc is an essential micronutrient for plants but can be highly toxic when present at excessive concentration. We aimed at investigation of detoxification mechanisms of maize plants (Zea mays L.) treated with the following combinations of zinc(II) and cadmium(II) ions 0 mM Zn2+ + 0 mM Cd2+; 100 mM Zn2+ + 0 mM Cd2+; 0 mM Zn2+ + 100 mM Cd2+; 10 mM Zn2+ + 100 mM Cd2+; 50 mM Zn2+ + 100 mM Cd2+; 75 mM Zn2+ + 100 mM Cd2+ and 100 mM Zn2+ + 100 mM Cd2+ for 10 days. In addition to growth parameters, we electrochemically determined the metal content in plants. To determine the antioxidant activity, we chose three methods FRAP, DPPH and ABTS, which differ in the spectrum of molecules with possible antioxidant activity. Pearson's correlation coefficient was found by comparison of FRAP and ABTS methods - rP = 0.7967 (Bradford) and 0.7957 (Pyrogallol Red). Pearson's correlation coefficients indicates mutual correlation of both DPPH and ABTS methods - rP = 0.8292 (Bradford) and 0.8737 (Pyrogallol Red). The highest correlation of Pearson's coefficients was found in comparison between DPPH and FRAP methods (0.9407 -- Bradford and 0.9211 Pyrogallol Red), suggesting a very high dependence of the two methods. Thus, our attention was focused on the determination of activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). We were also focused on the monitoring of the GST activity. In addition, we monitored its correlation with total content of thiol compounds (-SH) measured spectrometrically using Ellman's method. Content of thiol compounds, such as reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatins (PC2, PC5) was determined by HPLC--ED. The hi
dcterms:title
Study of cadmium and zinc interaction in maize Study of cadmium and zinc interaction in maize
skos:prefLabel
Study of cadmium and zinc interaction in maize Study of cadmium and zinc interaction in maize
skos:notation
RIV/62156489:43210/11:00180359!RIV12-MSM-43210___
n9:predkladatel
n10:orjk%3A43210
n4:aktivita
n16:S
n4:aktivity
S
n4:dodaniDat
n8:2012
n4:domaciTvurceVysledku
n15:8740658 n15:8594716 n15:9600426 n15:6218482 n15:7333323 n15:2528630 n15:2307049 n15:4995775 n15:8381038
n4:druhVysledku
n14:D
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n21:predkladatel
n4:idSjednocenehoVysledku
233037
n4:idVysledku
RIV/62156489:43210/11:00180359
n4:jazykVysledku
n11:eng
n4:klicovaSlova
heavy metals; interaction; trace elements
n4:klicoveSlovo
n5:trace%20elements n5:heavy%20metals n5:interaction
n4:kontrolniKodProRIV
[2958F4DE1019]
n4:mistoKonaniAkce
Brno
n4:mistoVydani
Brno
n4:nazevZdroje
MendelNet 2011
n4:obor
n17:CB
n4:pocetDomacichTvurcuVysledku
9
n4:pocetTvurcuVysledku
9
n4:rokUplatneniVysledku
n8:2011
n4:tvurceVysledku
Babula, Petr Dočekalová, Hana Adam, Vojtěch Zítka, Ondřej Kryštofová, Olga Kleckerová, Andrea Šobrová, Pavlína Sochor, Jiří Kizek, René
n4:typAkce
n18:CST
n4:zahajeniAkce
2011-01-01+01:00
s:numberOfPages
10
n20:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n3:isbn
978-80-7375-563-8
n6:organizacniJednotka
43210