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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F11%3A00180218%21RIV15-MSM-43210___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf
dcterms:description
Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protective mechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable tool for determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protective mechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable tool for determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2.
dcterms:title
A novel method for analysis of plants' resistance to heavy metals A novel method for analysis of plants' resistance to heavy metals
skos:prefLabel
A novel method for analysis of plants' resistance to heavy metals A novel method for analysis of plants' resistance to heavy metals
skos:notation
RIV/62156489:43210/11:00180218!RIV15-MSM-43210___
n6:aktivita
n18:S
n6:aktivity
S
n6:dodaniDat
n10:2015
n6:domaciTvurceVysledku
n7:2307049 n7:7333323 n7:4995775 n7:6218482 n7:3955397 n7:2645572 n7:8740658
n6:druhVysledku
n21:D
n6:duvernostUdaju
n11:S
n6:entitaPredkladatele
n20:predkladatel
n6:idSjednocenehoVysledku
184021
n6:idVysledku
RIV/62156489:43210/11:00180218
n6:jazykVysledku
n19:eng
n6:klicovaSlova
phytochelatin synthese; coulometric detection; heavy metals
n6:klicoveSlovo
n12:heavy%20metals n12:coulometric%20detection n12:phytochelatin%20synthese
n6:kontrolniKodProRIV
[C1C6F64FBB0D]
n6:mistoKonaniAkce
Brno
n6:mistoVydani
Brno
n6:nazevZdroje
MendelNet 2011
n6:obor
n8:GE
n6:pocetDomacichTvurcuVysledku
7
n6:pocetTvurcuVysledku
7
n6:rokUplatneniVysledku
n10:2011
n6:tvurceVysledku
Zítka, Ondřej Šobrová, Pavlína Zehnálek, Josef Beklová, Miroslava Kryštofová, Olga Adam, Vojtěch Kizek, René
n6:typAkce
n15:CST
n6:wos
341783400130
n6:zahajeniAkce
2011-01-01+01:00
s:numberOfPages
5
n16:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n13:isbn
978-80-7375-563-8
n9:organizacniJednotka
43210