This HTML5 document contains 53 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
n19http://linked.opendata.cz/ontology/domain/vavai/riv/typAkce/
dctermshttp://purl.org/dc/terms/
n22http://purl.org/net/nknouf/ns/bibtex#
n20http://localhost/temp/predkladatel/
n10http://linked.opendata.cz/resource/domain/vavai/projekt/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n18http://linked.opendata.cz/resource/domain/vavai/subjekt/
n17http://linked.opendata.cz/ontology/domain/vavai/
n21https://schema.org/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n15http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62156489%3A43210%2F11%3A00174192%21RIV12-GA0-43210___/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n8http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n14http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n9http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F11%3A00174192%21RIV12-GA0-43210___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitable tool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The lowest concentration of the toxic metal ions caused almost three times enhancing PCS activity compared to control samples. Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitable tool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The lowest concentration of the toxic metal ions caused almost three times enhancing PCS activity compared to control samples.
dcterms:title
Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity
skos:prefLabel
Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity
skos:notation
RIV/62156489:43210/11:00174192!RIV12-GA0-43210___
n17:predkladatel
n18:orjk%3A43210
n4:aktivita
n11:P
n4:aktivity
P(GA522/07/0692), P(GD204/09/H002)
n4:dodaniDat
n9:2012
n4:domaciTvurceVysledku
n7:4995775 n7:2645572 n7:2307049 n7:6218482 n7:7333323 n7:8740658 n7:3955397
n4:druhVysledku
n13:D
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
197422
n4:idVysledku
RIV/62156489:43210/11:00174192
n4:jazykVysledku
n14:eng
n4:klicovaSlova
phytochelatin synthase; coulometric analysis; HPLC
n4:klicoveSlovo
n8:phytochelatin%20synthase n8:HPLC n8:coulometric%20analysis
n4:kontrolniKodProRIV
[C7DD46FF9C9A]
n4:mistoKonaniAkce
MENDELU
n4:mistoVydani
Brno
n4:nazevZdroje
XI. pracovní setkání fyzikálních chemiků a elektrochemiků
n4:obor
n5:CB
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
7
n4:projekt
n10:GD204%2F09%2FH002 n10:GA522%2F07%2F0692
n4:rokUplatneniVysledku
n9:2011
n4:tvurceVysledku
Kryštofová, Olga Zehnálek, Josef Adam, Vojtěch Beklová, Miroslava Šobrová, Pavlína Zítka, Ondřej Kizek, René
n4:typAkce
n19:CST
n4:zahajeniAkce
2011-01-01+01:00
s:numberOfPages
4
n22:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n21:isbn
978-80-7375-514-0
n20:organizacniJednotka
43210