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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F11%3A00173510%21RIV13-GA0-43210___
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Phenols are broadly distributed in the plant kingdom and are the most abundant secondary metabolites of plants. Plant polyphenols have drawn increasing attention due to their potential antioxidant properties and their marked effects in the prevention of various oxidative stress-associated diseases such as cancer. The objective of this study was to investigate a suitable method for determination of protocatechuic acid, 4-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferulic acid, quercetin, resveratrol and quercitrin from apricot samples. A high-performance liquid chromatograph with electrochemical and UV detectors was used. The method was optimized in respect to both the separation selectivity of individual phenolic compounds and the maximum sensitivity with the electrochemical detection. The lowest limits of detection (3 S/N) using UV detection were estimated for ferulic acid (3 uM), quercitrin (4 uM) and quercetin (4 uM). By electrochemical detection values of 27 nM, 40 nM and 37 nM were achieved for ferulic acid, quercitrin and quercetin, respectively. It follows from the acquired results that the coulometric detection under a universal potential of 600 mV is more suitable and sensitive for polyphenol determination than UV detection at a universal wavelength of 260 nm. Subsequently, we tested the influence of solvent composition, vortexing and sonication on separation efficiency. Our results showed that a combination of water, acetone and methanol in a 20:20:60 ratio was the most effective for p-aminobenzoic acid, chlorgenic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, resveratrol and quercetin, in comparison with other solvents. On the other hand, vortexing at 4 °C produced the highest yield. Moreover, we tested the contents of individual polyphenols in the apricot cultivars Mamaria, Mold and LE-1075. The major phenolic compounds were chlorgenic acid and rutin. Chlorgenic acid was found in amounts of 2,302 Phenols are broadly distributed in the plant kingdom and are the most abundant secondary metabolites of plants. Plant polyphenols have drawn increasing attention due to their potential antioxidant properties and their marked effects in the prevention of various oxidative stress-associated diseases such as cancer. The objective of this study was to investigate a suitable method for determination of protocatechuic acid, 4-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferulic acid, quercetin, resveratrol and quercitrin from apricot samples. A high-performance liquid chromatograph with electrochemical and UV detectors was used. The method was optimized in respect to both the separation selectivity of individual phenolic compounds and the maximum sensitivity with the electrochemical detection. The lowest limits of detection (3 S/N) using UV detection were estimated for ferulic acid (3 uM), quercitrin (4 uM) and quercetin (4 uM). By electrochemical detection values of 27 nM, 40 nM and 37 nM were achieved for ferulic acid, quercitrin and quercetin, respectively. It follows from the acquired results that the coulometric detection under a universal potential of 600 mV is more suitable and sensitive for polyphenol determination than UV detection at a universal wavelength of 260 nm. Subsequently, we tested the influence of solvent composition, vortexing and sonication on separation efficiency. Our results showed that a combination of water, acetone and methanol in a 20:20:60 ratio was the most effective for p-aminobenzoic acid, chlorgenic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, resveratrol and quercetin, in comparison with other solvents. On the other hand, vortexing at 4 °C produced the highest yield. Moreover, we tested the contents of individual polyphenols in the apricot cultivars Mamaria, Mold and LE-1075. The major phenolic compounds were chlorgenic acid and rutin. Chlorgenic acid was found in amounts of 2,302
dcterms:title
Comparison of various easy-to-use procedures for extraction of phenols from apricot fruits Comparison of various easy-to-use procedures for extraction of phenols from apricot fruits
skos:prefLabel
Comparison of various easy-to-use procedures for extraction of phenols from apricot fruits Comparison of various easy-to-use procedures for extraction of phenols from apricot fruits
skos:notation
RIV/62156489:43210/11:00173510!RIV13-GA0-43210___
n15:predkladatel
n17:orjk%3A43210
n3:aktivita
n6:P n6:V
n3:aktivity
P(GA522/07/0692), P(QI91A032), V
n3:cisloPeriodika
4
n3:dodaniDat
n7:2013
n3:domaciTvurceVysledku
n4:9099107 n4:3955397 n4:7333323 n4:2307049 n4:8082146 n4:8740658 n4:8594716 n4:2645572 n4:4995775
n3:druhVysledku
n10:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
191308
n3:idVysledku
RIV/62156489:43210/11:00173510
n3:jazykVysledku
n19:eng
n3:klicovaSlova
phenol; extraction; limits of detection
n3:klicoveSlovo
n5:phenol n5:extraction n5:limits%20of%20detection
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[877026EB9242]
n3:nazevZdroje
Molecules
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
10
n3:projekt
n8:QI91A032 n8:GA522%2F07%2F0692
n3:rokUplatneniVysledku
n7:2011
n3:svazekPeriodika
16
n3:tvurceVysledku
Beklová, Miroslava Kizek, René Zehnálek, Josef Zítka, Ondřej Šobrová, Pavlína Adam, Vojtěch Krška, Boris Skaličková, Sylvie Sochor, Jiří Rop, Otakar
s:issn
1420-3049
s:numberOfPages
23
n16:organizacniJednotka
43210