This HTML5 document contains 39 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n7http://localhost/temp/predkladatel/
n18http://linked.opendata.cz/resource/domain/vavai/projekt/
n4http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n10http://linked.opendata.cz/resource/domain/vavai/subjekt/
n9http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n15http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62156489%3A43210%2F11%3A00166608%21RIV12-GA0-43210___/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n8http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F11%3A00166608%21RIV12-GA0-43210___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18 has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets. The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18 has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets.
dcterms:title
The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR
skos:prefLabel
The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR
skos:notation
RIV/62156489:43210/11:00166608!RIV12-GA0-43210___
n9:predkladatel
n10:orjk%3A43210
n3:aktivita
n16:P
n3:aktivity
P(GA523/09/0844)
n3:cisloPeriodika
5
n3:dodaniDat
n8:2012
n3:domaciTvurceVysledku
n4:8551472 n4:2529157
n3:druhVysledku
n17:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
198346
n3:idVysledku
RIV/62156489:43210/11:00166608
n3:jazykVysledku
n12:eng
n3:klicovaSlova
reference gene; pig
n3:klicoveSlovo
n6:pig n6:reference%20gene
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[CF11B43FD9BE]
n3:nazevZdroje
Czech Journal of Animal Science
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n18:GA523%2F09%2F0844
n3:rokUplatneniVysledku
n8:2011
n3:svazekPeriodika
56
n3:tvurceVysledku
Nesvadbová, Michaela Knoll, Aleš
s:issn
1212-1819
s:numberOfPages
4
n7:organizacniJednotka
43210