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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F10%3A00169162%21RIV12-GA0-43210___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Studying of metal ions effects on plant cells and whole plants are still topical. Early somatic embryos (ESEs) of spruce represent the unique plant model system, which can be used to study of various types of environmental stresses including metal ions under well controlled experimental conditions. In the paper nuclear magnetic resonance (NMR) and electrochemical techniques were employed to investigate the effect of cadmium(II) ions (0, 50, 150, 250, 500 and 1,000 mu M) on early somatic embryos (ESEs) of Norway spruce, clone 2/32. The ESEs were treated with cadmium(II) ions for fourteen days. The increase in cluster area and viability were determined by image analysis and fluorescein diacetate/propidium iodide double staining, respectively. Growth characteristics revealed the ESEs treated with cadmium-ethylene diamine tetra-acetic acid (Cd-EDTA) showed an increase in growth-inhibiting effect due to cadmium(II). Furthermore, total thiol content and certain low molecular mass plant thiols (cysteine, reduced glutathione, oxidized glutathione and phytochelatin2) were determined using Brdicka reaction and liquid chromatography with electrochemical detection, respectively. The highest level of phytochelatin2 synthesis was determined in ESEs treated with 50 mu M Cd-EDTA. The average content of phytochelatin2 varied from 850 to 1800 nM/g FW. We also attempted to determine changes in water content in the ESEs treated with cadmium(II) ions using NMR. The highest increase in water content was observed at clusters treated with 250 mu M Cd-EDTA. The clusters treated with higher cadmium concentrations (more than 250 mu M) demonstrate only slow increase in the water content. Water metabolism at woody plants and their embryos exposed to cadmium(II) ions has not yet been described. Based on the results obtained it can conclude that the cells of embryonic clusters attempted to excrete cadmium(II) ions or, more precisely, decrease its concentration within the cluster. This phenomenon Studying of metal ions effects on plant cells and whole plants are still topical. Early somatic embryos (ESEs) of spruce represent the unique plant model system, which can be used to study of various types of environmental stresses including metal ions under well controlled experimental conditions. In the paper nuclear magnetic resonance (NMR) and electrochemical techniques were employed to investigate the effect of cadmium(II) ions (0, 50, 150, 250, 500 and 1,000 mu M) on early somatic embryos (ESEs) of Norway spruce, clone 2/32. The ESEs were treated with cadmium(II) ions for fourteen days. The increase in cluster area and viability were determined by image analysis and fluorescein diacetate/propidium iodide double staining, respectively. Growth characteristics revealed the ESEs treated with cadmium-ethylene diamine tetra-acetic acid (Cd-EDTA) showed an increase in growth-inhibiting effect due to cadmium(II). Furthermore, total thiol content and certain low molecular mass plant thiols (cysteine, reduced glutathione, oxidized glutathione and phytochelatin2) were determined using Brdicka reaction and liquid chromatography with electrochemical detection, respectively. The highest level of phytochelatin2 synthesis was determined in ESEs treated with 50 mu M Cd-EDTA. The average content of phytochelatin2 varied from 850 to 1800 nM/g FW. We also attempted to determine changes in water content in the ESEs treated with cadmium(II) ions using NMR. The highest increase in water content was observed at clusters treated with 250 mu M Cd-EDTA. The clusters treated with higher cadmium concentrations (more than 250 mu M) demonstrate only slow increase in the water content. Water metabolism at woody plants and their embryos exposed to cadmium(II) ions has not yet been described. Based on the results obtained it can conclude that the cells of embryonic clusters attempted to excrete cadmium(II) ions or, more precisely, decrease its concentration within the cluster. This phenomenon
dcterms:title
Effects of Cadmium(II) Ions on Early Somatic Embryos of Norway spruce Studied by Using Electrochemical Techniques and Nuclear Magnetic Resonance Effects of Cadmium(II) Ions on Early Somatic Embryos of Norway spruce Studied by Using Electrochemical Techniques and Nuclear Magnetic Resonance
skos:prefLabel
Effects of Cadmium(II) Ions on Early Somatic Embryos of Norway spruce Studied by Using Electrochemical Techniques and Nuclear Magnetic Resonance Effects of Cadmium(II) Ions on Early Somatic Embryos of Norway spruce Studied by Using Electrochemical Techniques and Nuclear Magnetic Resonance
skos:notation
RIV/62156489:43210/10:00169162!RIV12-GA0-43210___
n3:aktivita
n15:V n15:Z n15:S n15:P
n3:aktivity
P(1M06030), P(GA102/08/1546), P(GA522/07/0692), P(GD204/09/H002), S, V, Z(AV0Z20650511), Z(MSM6215712402)
n3:cisloPeriodika
11
n3:dodaniDat
n9:2012
n3:domaciTvurceVysledku
n5:6218482 n5:3955397 n5:9000240 n5:8740658 n5:2645572 n5:2307049 n5:9600426 n5:4995775 n5:1249940
n3:druhVysledku
n16:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
256285
n3:idVysledku
RIV/62156489:43210/10:00169162
n3:jazykVysledku
n18:eng
n3:klicovaSlova
electrochemistry; early somatic embryos; cadmium
n3:klicoveSlovo
n4:early%20somatic%20embryos n4:cadmium n4:electrochemistry
n3:kodStatuVydavatele
RS - Srbská republika
n3:kontrolniKodProRIV
[DC48BE00A22E]
n3:nazevZdroje
International Journal of Electrochemical Science
n3:obor
n10:CG
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
10
n3:projekt
n6:GA102%2F08%2F1546 n6:GA522%2F07%2F0692 n6:GD204%2F09%2FH002 n6:1M06030
n3:rokUplatneniVysledku
n9:2010
n3:svazekPeriodika
5
n3:tvurceVysledku
Bartůšek, Karel Babula, Petr Havel, Ladislav Zítka, Ondřej Zehnálek, Josef Húska, Dalibor Kryštofová, Olga Adam, Vojtěch Beklová, Miroslava Kizek, René
n3:zamer
n8:AV0Z20650511 n8:MSM6215712402
s:issn
1452-3981
s:numberOfPages
15
n19:organizacniJednotka
43210