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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F10%3A00166772%21RIV12-GA0-43210___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Prostate cancer (PCa) is one of the most frequent cancer and one of the most frequent cancerrelated cause of death among men. Therefore, early diagnosis, differentiation between risky and relative benign forms and understanding of pathogenesis of disease for further therapeutic approaches is highly desirable. Healthy prostate is unique in zinc accumulation. Zinc is (mostly) buffered by cysteine-rich low molecular protein metallothionein (MT). In contrast, PCa has altered zinc metabolism and elevated MT. In PCa patients, MT is elevated even in serum and can therefore be used as potential tumor marker due to high specifity to PCa. This could be very desirable because of inaccuracies of current prostatic specific antigen (PSA) screening. The aims of this study is (1) to analyze MT-zinc relation on cell lines: to determine zinc and MT levels in cell lines PC-3 (cancer) and PNT1A (control), (2) to find relations between MT and PSA, (3) to describe potential effects of MT and/or zinc on prostate cancer pathogenesis, (4) to determine serum MT level,(5) to find relations between MT level and patient's disease grading. We used (1) optimized fully automated immunochemical methods for detection of serum PSA in serum, (2) protein separation with paramagnetic microparticles modified with antibody against PSA and MT, (3) PAGE gel silver and coomassie staining and colorimetric detection. We found (1) statistically significant (p=0.001) MT elevation in PCa lines and in PCa serum, (2) significant PSA elevation in cell lines, (3) strong correlation between intracel. zinc and MT, (4) no correlation between disease grading/patient's history, PSA level and MT level. We found MT/zinc play a role in PCa pathogenesis, further understanding may have therapeutic implications. By our findings, MT is a good candidate for new marker for PCa screening, developing of automated diagnostic methods is highly desirable. Prostate cancer (PCa) is one of the most frequent cancer and one of the most frequent cancerrelated cause of death among men. Therefore, early diagnosis, differentiation between risky and relative benign forms and understanding of pathogenesis of disease for further therapeutic approaches is highly desirable. Healthy prostate is unique in zinc accumulation. Zinc is (mostly) buffered by cysteine-rich low molecular protein metallothionein (MT). In contrast, PCa has altered zinc metabolism and elevated MT. In PCa patients, MT is elevated even in serum and can therefore be used as potential tumor marker due to high specifity to PCa. This could be very desirable because of inaccuracies of current prostatic specific antigen (PSA) screening. The aims of this study is (1) to analyze MT-zinc relation on cell lines: to determine zinc and MT levels in cell lines PC-3 (cancer) and PNT1A (control), (2) to find relations between MT and PSA, (3) to describe potential effects of MT and/or zinc on prostate cancer pathogenesis, (4) to determine serum MT level,(5) to find relations between MT level and patient's disease grading. We used (1) optimized fully automated immunochemical methods for detection of serum PSA in serum, (2) protein separation with paramagnetic microparticles modified with antibody against PSA and MT, (3) PAGE gel silver and coomassie staining and colorimetric detection. We found (1) statistically significant (p=0.001) MT elevation in PCa lines and in PCa serum, (2) significant PSA elevation in cell lines, (3) strong correlation between intracel. zinc and MT, (4) no correlation between disease grading/patient's history, PSA level and MT level. We found MT/zinc play a role in PCa pathogenesis, further understanding may have therapeutic implications. By our findings, MT is a good candidate for new marker for PCa screening, developing of automated diagnostic methods is highly desirable.
dcterms:title
Metallothionein - Zinc- Prostate cancer: Pathogenesis and diagnostic use Metallothionein - Zinc- Prostate cancer: Pathogenesis and diagnostic use
skos:prefLabel
Metallothionein - Zinc- Prostate cancer: Pathogenesis and diagnostic use Metallothionein - Zinc- Prostate cancer: Pathogenesis and diagnostic use
skos:notation
RIV/62156489:43210/10:00166772!RIV12-GA0-43210___
n3:aktivita
n11:S n11:P
n3:aktivity
P(GP301/09/P436), S
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n10:2307049 n10:4995775 n10:8740658 n10:3931315 n10:9600426 n10:9584366
n3:druhVysledku
n9:D
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
270615
n3:idVysledku
RIV/62156489:43210/10:00166772
n3:jazykVysledku
n7:eng
n3:klicovaSlova
matalothionein; prostate cancer; zinc
n3:klicoveSlovo
n12:prostate%20cancer n12:zinc n12:matalothionein
n3:kontrolniKodProRIV
[E31574E89FE0]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
MendelNet 2010 Proceedings of International Ph.D. Students Conference
n3:obor
n21:CE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
7
n3:projekt
n5:GP301%2F09%2FP436
n3:rokUplatneniVysledku
n14:2010
n3:tvurceVysledku
Adam, Vojtěch Babula, Petr Zítka, Ondřej Masařík, Michal Cernei, Natalia Vladimirovna Kizek, René Gumulec, Jaromír
n3:typAkce
n13:EUR
n3:zahajeniAkce
2000-01-01+01:00
s:numberOfPages
7
n4:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n18:isbn
978-80-7375-453-2
n8:organizacniJednotka
43210