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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F10%3A00166433%21RIV11-GA0-43210___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
A method that is easy to use, rapid, with a low cost of detecting viral nucleic acid in a biological sample represents the essential tool in targeted therapy. In this study, we report the use of paramagnetic microparticles covered by streptavidin and modified by an oligonucleotide probe with a specific viral sequence labeled by biotin to detect human immunodeficiency virus (HIV) and influenza virus subtype H5N1. The viral nucleic acids were primarily detected by adsorptive transfer stripping technique coupled with square wave voltammetry using carbon paste, hanging mercury drop or carbon nanotubes-based screen-printed working electrodes. Detection limits were estimated for both sequences down to picograms per 3 ul. To isolate the viral sequences, paramagnetic microparticles covered with biotin-labeled oligonucleotides were used. We calculated the yield of isolation for H5N1 and/or HIV sequences, which was defined as %22isolated concentration of viral nucleic acid sequence%22/%22given viral nucleic acid sequ A method that is easy to use, rapid, with a low cost of detecting viral nucleic acid in a biological sample represents the essential tool in targeted therapy. In this study, we report the use of paramagnetic microparticles covered by streptavidin and modified by an oligonucleotide probe with a specific viral sequence labeled by biotin to detect human immunodeficiency virus (HIV) and influenza virus subtype H5N1. The viral nucleic acids were primarily detected by adsorptive transfer stripping technique coupled with square wave voltammetry using carbon paste, hanging mercury drop or carbon nanotubes-based screen-printed working electrodes. Detection limits were estimated for both sequences down to picograms per 3 ul. To isolate the viral sequences, paramagnetic microparticles covered with biotin-labeled oligonucleotides were used. We calculated the yield of isolation for H5N1 and/or HIV sequences, which was defined as %22isolated concentration of viral nucleic acid sequence%22/%22given viral nucleic acid sequ
dcterms:title
Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes
skos:prefLabel
Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes
skos:notation
RIV/62156489:43210/10:00166433!RIV11-GA0-43210___
n3:aktivita
n18:P
n3:aktivity
P(GA102/08/1546), P(KAN208130801)
n3:cisloPeriodika
3
n3:dodaniDat
n8:2011
n3:domaciTvurceVysledku
n5:2307049 n5:9000240 n5:4995775 n5:4489705
n3:druhVysledku
n4:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
255657
n3:idVysledku
RIV/62156489:43210/10:00166433
n3:jazykVysledku
n16:eng
n3:klicovaSlova
voltammetry; microparticles; nucleic acid
n3:klicoveSlovo
n6:microparticles n6:nucleic%20acid n6:voltammetry
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[BDE36F22B106]
n3:nazevZdroje
Microfluidics and Nanofluidics
n3:obor
n13:CG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n10:GA102%2F08%2F1546 n10:KAN208130801
n3:rokUplatneniVysledku
n8:2010
n3:svazekPeriodika
8
n3:tvurceVysledku
Hubálek, Jaromír Kizek, René Adam, Vojtěch Húska, Dalibor
s:issn
1613-4982
s:numberOfPages
11
n17:organizacniJednotka
43210