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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F09%3A00161014%21RIV11-MSM-43210___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
To overcome the problematic detection Bean yellow mosaic virus (BYMV) in gladiolus corm and cormlets, we tested suitability of different diagnostic methods such as ELISA, one step RT-PCR and IC-PCR. Among these methods, DAS-ELISA and one step RT-PCR was able to detect in leaves and failed to detect in corms or cormlets whereas IC-RT-PCR method was very sensitive and able to detect virus in both gladiolus leaves and corm or cormlets. To overcome the problematic detection Bean yellow mosaic virus (BYMV) in gladiolus corm and cormlets, we tested suitability of different diagnostic methods such as ELISA, one step RT-PCR and IC-PCR. Among these methods, DAS-ELISA and one step RT-PCR was able to detect in leaves and failed to detect in corms or cormlets whereas IC-RT-PCR method was very sensitive and able to detect virus in both gladiolus leaves and corm or cormlets.
dcterms:title
Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus
skos:prefLabel
Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus
skos:notation
RIV/62156489:43210/09:00161014!RIV11-MSM-43210___
n4:aktivita
n13:Z
n4:aktivity
Z(MSM6215648905)
n4:cisloPeriodika
3
n4:dodaniDat
n10:2011
n4:domaciTvurceVysledku
Duraisamy, Ganesh Selvaraj n17:4761790 n17:1517015
n4:druhVysledku
n16:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n8:predkladatel
n4:idSjednocenehoVysledku
307635
n4:idVysledku
RIV/62156489:43210/09:00161014
n4:jazykVysledku
n18:eng
n4:klicovaSlova
Gladiolus; Bean yellow mosaic virus; ELISA; RT-PCR; IC-RT-PCR
n4:klicoveSlovo
n11:Gladiolus n11:Bean%20yellow%20mosaic%20virus n11:RT-PCR n11:ELISA n11:IC-RT-PCR
n4:kodStatuVydavatele
BE - Belgické království
n4:kontrolniKodProRIV
[9B8CC9429BDF]
n4:nazevZdroje
Communications in Agricultural and Applied Biological Sciences
n4:obor
n5:GF
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:rokUplatneniVysledku
n10:2009
n4:svazekPeriodika
74
n4:tvurceVysledku
Holková, Ludmila Pokorný, Radovan Duraisamy, Ganesh Selvaraj
n4:zamer
n7:MSM6215648905
s:issn
1379-1176
s:numberOfPages
7
n9:organizacniJednotka
43210