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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F09%3A00139259%21RIV09-GA0-43210___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Cílem práce bylo studium polymorfismu mikrosatelitních markerů na 3H a 7H chromozomu ječmene u genotypů ječmene rezistentních a náchylných k Rhynchosporium secalis. Soubor patnácti analyzovaných genotypů ječmene setého (Hordeum vulgare L.) jarní formy pochází z kolekce genetických zdrojů Zemědělského výzkumného ústavu Kroměříž, s.r.o.: Abyssinian, Atlas, Atlas 46, Atlas 57, Bay, Cambrinus, Clipper, Jet, Kitchin, Kompakt, Korál, La Mesita, Nigrinudum Abyssinum, Psaknon a Rapid. K analýzám bylo vybráno celkem 33 SSR markerů, z nichž 17 bylo lokalizováno na 3H chromozomu ječmene a 16 SSR markerů bylo lokalizováno na 7H chromozomu ječmene. Mikrosatelitní markery byly vybrány z databáze GrainGenes a z literatury, kde je uvedena sekvence primerů, motiv a lokalizace. Celková genomická DNA byla izolována pomocí izolačního kitu DNeasy Plant Mini Kit od firmy Qiagen (GE) z listů rostlin ve stáří 6--7 dní. Koncentrace izolované DNA byla zjišťována spektrofotometricky. Výsledné produkty PCR reakce byly elektrofor The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgare L.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Kroměříž, Ltd. showing various degrees of susceptibility to Rhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes against Rhynchosporium secalis is localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one marker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgare L.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Kroměříž, Ltd. showing various degrees of susceptibility to Rhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes against Rhynchosporium secalis is localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one marker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the
dcterms:title
Polymorfismus mikrosatelitních markerů na 3H a 7H chromozomu u genotypů ječmene rezistentních a náchylných k Rhynchosporium secalis Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis
skos:prefLabel
Polymorfismus mikrosatelitních markerů na 3H a 7H chromozomu u genotypů ječmene rezistentních a náchylných k Rhynchosporium secalis Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis
skos:notation
RIV/62156489:43210/09:00139259!RIV09-GA0-43210___
n3:aktivita
n14:P
n3:aktivity
P(GA521/03/0938)
n3:cisloPeriodika
2
n3:dodaniDat
n8:2009
n3:domaciTvurceVysledku
n4:6512348 n4:1980963 n4:9220542
n3:druhVysledku
n11:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
334183
n3:idVysledku
RIV/62156489:43210/09:00139259
n3:jazykVysledku
n9:eng
n3:klicovaSlova
microsatellite markers; resistance; polymorphism; Rhynchosporium secalis
n3:klicoveSlovo
n12:microsatellite%20markers n12:resistance n12:polymorphism n12:Rhynchosporium%20secalis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[6CB1888228B2]
n3:nazevZdroje
Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis : Acta of Mendel University of agriculture and forestry Brno = Acta Mendelovy zemědělské a lesnické univerzity v Brně
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n7:GA521%2F03%2F0938
n3:rokUplatneniVysledku
n8:2009
n3:svazekPeriodika
LVII
n3:tvurceVysledku
Vyhnánek, Tomáš Nevimová, Hana Bednář, Jan
s:issn
1211-8516
s:numberOfPages
10
n13:organizacniJednotka
43210