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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F08%3A9P000445%21RIV09-MZE-43210___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattle and the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentioned reasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed using on line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1). Pomocí AS-PCR byla detekována mutace C313Y u plemene gasconne. Mutace je odpovědná za dvojite osvaleni u tohoto plemene. Důvěryhodnost detekce pomoci nové metody byla ověřena sekvenovanim daného úseku. In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattle and the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentioned reasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed using on line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1).
dcterms:title
Novel detection of c131y mutation using allele specific PCR (AS – PCR) Novel detection of c131y mutation using allele specific PCR (AS – PCR) Nový způsob detekce mutace C313Y pomocí alelle specific PCR (AS-PCR)
skos:prefLabel
Novel detection of c131y mutation using allele specific PCR (AS – PCR) Novel detection of c131y mutation using allele specific PCR (AS – PCR) Nový způsob detekce mutace C313Y pomocí alelle specific PCR (AS-PCR)
skos:notation
RIV/62156489:43210/08:9P000445!RIV09-MZE-43210___
n3:aktivita
n14:P
n3:aktivity
P(1G58073), P(2B08037)
n3:cisloPeriodika
1
n3:dodaniDat
n4:2009
n3:domaciTvurceVysledku
n12:6906311
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
383262
n3:idVysledku
RIV/62156489:43210/08:9P000445
n3:jazykVysledku
n10:eng
n3:klicovaSlova
MSTN; Gasconne; AS-PCR
n3:klicoveSlovo
n7:Gasconne n7:AS-PCR n7:MSTN
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[0CCE829B2109]
n3:nazevZdroje
Journal of Agrobiology
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:projekt
n6:1G58073 n6:2B08037
n3:rokUplatneniVysledku
n4:2008
n3:svazekPeriodika
25
n3:tvurceVysledku
Stehlík, Libor
s:issn
1803-4403
s:numberOfPages
3
n18:organizacniJednotka
43210