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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F08%3A00133932%21RIV09-MSM-43210___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Heavy metals are considered to be dangerous pollutants due to their toxic effects to all organisms. Most of the methods used for remediation of polluted environment suffer from high costs. One of the alternative way represents phytoremediation [1]. This process describes the treatment of environmental problems (bioremediation) through the use of plants. When heavy metals enter into a plant cell, they initiate the biosynthesis of proteins rich in sulphur called phytochelatins, which are used for heavy metal detoxification. Phytochelatins (PC) are cysteine-rich small peptides consist of 4-23 amino acids, which have a basic formula (Glu-Cys)n-Gly (n = 2 to 11). The synthesis of phytochelatins proceeds from glutahione by transferring a Glu-Cys moiety from a donor to an acceptor molecule. The reaction is catalyzed by Glu-Cys dipeptidyl transpeptidase (EC 2.3.2.15), which has been called as phytochelatin synthase (PCS). In vitro the activity of the partially purified enzyme was active only in the presence Těžké kovy jsou považovány za nebezpečné látky znečišťující všechny organismů. Většina metod používaných pro sanace znečištěného životního prostředí trpí vysokými náklady. Jedním z alternativní způsob představuje Fytoremediace [1]. Tento proces popisuje léčení problémů týkajících se životního prostředí (Bioremediace) prostřednictvím využití rostlin. Rostliny přijímají těžké kovy a zahajují biosyntézu bílkovin bohatých na SH nazývaných phytochelatiny, které se používají pro detoxikaci těžkých kovů. Phytochelatins (PC) jsou bohaté na cystein-malé peptidy se skládají z 4-23 aminokyselin, které mají základní vzorec (Glu-Cys) n-Gly (n = 2 až 11). Syntéza phytochelatins vychází z kondenzace několika molekul glutahionu. Tato reakce je catalyzed Glu-Cys dipeptidyl transpeptidase (ES 2.3.2.15), která byla označována jako phytochelatin syntáza (PCS). In vitro aktivita částečně čištěný enzym byl aktivní pouze v přítomnosti iontů kovů. Nejlepší aktivátor PCS byl testován kadmium následuje stříbro, bismut, olovo Heavy metals are considered to be dangerous pollutants due to their toxic effects to all organisms. Most of the methods used for remediation of polluted environment suffer from high costs. One of the alternative way represents phytoremediation [1]. This process describes the treatment of environmental problems (bioremediation) through the use of plants. When heavy metals enter into a plant cell, they initiate the biosynthesis of proteins rich in sulphur called phytochelatins, which are used for heavy metal detoxification. Phytochelatins (PC) are cysteine-rich small peptides consist of 4-23 amino acids, which have a basic formula (Glu-Cys)n-Gly (n = 2 to 11). The synthesis of phytochelatins proceeds from glutahione by transferring a Glu-Cys moiety from a donor to an acceptor molecule. The reaction is catalyzed by Glu-Cys dipeptidyl transpeptidase (EC 2.3.2.15), which has been called as phytochelatin synthase (PCS). In vitro the activity of the partially purified enzyme was active only in the presence
dcterms:title
Analýza fytochelatinů a fytochelatin syntasy pomocí HPLC s elektrochemickou detekcí Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection
skos:prefLabel
Analýza fytochelatinů a fytochelatin syntasy pomocí HPLC s elektrochemickou detekcí Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection
skos:notation
RIV/62156489:43210/08:00133932!RIV09-MSM-43210___
n3:aktivita
n15:P
n3:aktivity
P(1M06030)
n3:dodaniDat
n13:2009
n3:domaciTvurceVysledku
n12:4995775 n12:8279314 n12:2307049 n12:2007398 n12:9600426 n12:1249940 n12:9000240
n3:druhVysledku
n11:D
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
355860
n3:idVysledku
RIV/62156489:43210/08:00133932
n3:jazykVysledku
n4:cze
n3:klicovaSlova
fytochelatins; HPLC; fytochelatinsynthasa
n3:klicoveSlovo
n7:HPLC n7:fytochelatins n7:fytochelatinsynthasa
n3:kontrolniKodProRIV
[38B2D49625C5]
n3:mistoKonaniAkce
Warsaw, Poland
n3:mistoVydani
Warsaw, Poland
n3:nazevZdroje
7th Workshop on Sulfur in Plants
n3:obor
n6:CE
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n21:1M06030
n3:rokUplatneniVysledku
n13:2008
n3:tvurceVysledku
Havel, Ladislav Diopan, Václav Adam, Vojtěch Babula, Petr Húska, Dalibor Baloun, Jiří Kizek, René
n3:typAkce
n10:WRD
n3:zahajeniAkce
2008-01-01+01:00
s:numberOfPages
1
n19:hasPublisher
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
n17:isbn
83-917432-8-4
n5:organizacniJednotka
43210