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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F08%3A00133454%21RIV09-AV0-43210___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one of the possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidized GSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. Finally, we utili Kuli vysoké reaktivitě SH-skupin glutathionu není překvapující, že glutathion může ovlivňovat léčbu nemocí včetně onkologických. V práci jsme se elektrochemickými metodami pokusili charakterizovat GSH a oxidovaný glutathion s ohledem na dosažení nejnižších detekčních limitů. Za nejvhodnějších podmínek jsme dosáhli limitů GSH/GSSG - 100 pg / ml a 50 ng / ml, resp. Pak byla zkoumána interakce GSH a cisplatiny a DNA. Průměrné množstvé léčebné cisplatiny navázané na 500 ng DNA byla 8,1 ng. Elektrochemická detekce představuje alternativní methodu pro stanovení biologicky aktivních sloučenin. Zde jsme ukázaly různé elektrochemické techniky jako vhodné nástroje pro sledování interakce mezi thioly, cytostatik a DNA. Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one of the possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidized GSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. Finally, we utili
dcterms:title
Elektroanalýza komplexu cisplatiny s glutathionem a interakce cisplatiny s DNA Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions
skos:prefLabel
Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions Elektroanalýza komplexu cisplatiny s glutathionem a interakce cisplatiny s DNA Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions
skos:notation
RIV/62156489:43210/08:00133454!RIV09-AV0-43210___
n3:aktivita
n10:P
n3:aktivity
P(IAA401990701)
n3:cisloPeriodika
1
n3:dodaniDat
n11:2009
n3:domaciTvurceVysledku
n7:4995775 n7:8740658 n7:2307049 n7:3790185 n7:9000240
n3:druhVysledku
n9:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
365759
n3:idVysledku
RIV/62156489:43210/08:00133454
n3:jazykVysledku
n8:eng
n3:klicovaSlova
glutathion; DNA; biointeraction
n3:klicoveSlovo
n4:biointeraction n4:glutathion n4:DNA
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[DEEAD23C5F7D]
n3:nazevZdroje
Toxicology Letters
n3:obor
n13:BO
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n16:IAA401990701
n3:rokUplatneniVysledku
n11:2008
n3:svazekPeriodika
180
n3:tvurceVysledku
Adam, Vojtěch Kizek, René Húska, Dalibor Zítka, Ondřej Trnková, Libuše
s:issn
0378-4274
s:numberOfPages
1
n12:organizacniJednotka
43210