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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F08%3A00122083%21RIV09-MSM-43210___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
In the present work we report on investigation of affecting of duckweed (Lemna minor) by various doses of cisplatin (0, 5, 10, 20, 40, 80 and 160 uM) for 4 days. The toxic influence of cisplatin was evaluated on the basis of growth inhibition expressed as number of leaves, growth rate, and total amount of biomass. The result value of 96hEC50, calculated from growth inhibition with comparison of growth rates, was 6.93 uM. Moreover we aimed on determination of cisplatin content using differential pulse voltammetry. The highest content of cisplatin (320 ng g-1 of fresh weight) was determined in plants treated by 80 uM at the second day of treatment. Plants protect themselves against heavy metals by means of synthesis of cysteine-rich peptides such as glutathione and phytochelatins. Thus thiol determination in the treated plants by means of Brdicka reaction followed. The marked increase in thiol concentration detected is associated with defence reaction of the plant against stress caused by cisplatin. V této práci jsme zkoumli vliv různých dávek cis-platiny (0-160uM) ma Lemna minor po dobu 4 dnů. Toxický vliv cis-platiny byl zjištěn na základě růstové inhibice vyjádřené v podobě počtu listů a růstového poměru byiomasy. Zjištěná hodnota pro poměr tvorby biomasy EC 50 po 96 hodinách byla 6.93 uM. Mimoto jsem zjistliy obsah cysplatiny pomocí diferenční pulsní voltametrie. Nejvyssi obsah byl cis-platiny 320 ng/g čerstvé hmotnosti byl zjištěn u rostlin vystavených 80 uM ve druhém dnu experimentu. Rostlinná chrana zahrnovala i tvorbu na cystein bohatých sloučebnin. In the present work we report on investigation of affecting of duckweed (Lemna minor) by various doses of cisplatin (0, 5, 10, 20, 40, 80 and 160 uM) for 4 days. The toxic influence of cisplatin was evaluated on the basis of growth inhibition expressed as number of leaves, growth rate, and total amount of biomass. The result value of 96hEC50, calculated from growth inhibition with comparison of growth rates, was 6.93 uM. Moreover we aimed on determination of cisplatin content using differential pulse voltammetry. The highest content of cisplatin (320 ng g-1 of fresh weight) was determined in plants treated by 80 uM at the second day of treatment. Plants protect themselves against heavy metals by means of synthesis of cysteine-rich peptides such as glutathione and phytochelatins. Thus thiol determination in the treated plants by means of Brdicka reaction followed. The marked increase in thiol concentration detected is associated with defence reaction of the plant against stress caused by cisplatin.
dcterms:title
Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry Vliv cisplatiny na vodní rostlinu Lemna minor tudovaný voltametrií
skos:prefLabel
Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry Vliv cisplatiny na vodní rostlinu Lemna minor tudovaný voltametrií Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry
skos:notation
RIV/62156489:43210/08:00122083!RIV09-MSM-43210___
n3:aktivita
n7:P n7:Z
n3:aktivity
P(1M06030), P(GA522/07/0692), Z(MSM6215712402)
n3:cisloPeriodika
1
n3:dodaniDat
n9:2009
n3:domaciTvurceVysledku
n5:8279314 n5:3955397 n5:2645572 n5:2307049 n5:4995775 n5:1249940 n5:3954382 n5:9000240
n3:druhVysledku
n4:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
355060
n3:idVysledku
RIV/62156489:43210/08:00122083
n3:jazykVysledku
n12:eng
n3:klicovaSlova
platin based cytostatic
n3:klicoveSlovo
n17:platin%20based%20cytostatic
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[06C202838C87]
n3:nazevZdroje
Bioelectrochemistry
n3:obor
n10:CE
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
12
n3:projekt
n13:GA522%2F07%2F0692 n13:1M06030
n3:rokUplatneniVysledku
n9:2008
n3:svazekPeriodika
72
n3:tvurceVysledku
Rauscherová, L. Pikula, Jiří Zehnálek, Josef Singer, Christoph Baloun, Jiří Kizek, René Adam, Vojtěch Beklová, Miroslava Havel, Ladislav Sures, Bernd Šupálková, Veronika Húska, Dalibor
n3:zamer
n14:MSM6215712402
s:issn
1567-5394
s:numberOfPages
7
n18:organizacniJednotka
43210