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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F08%3A00118570%21RIV08-MSM-43210___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Our work is focused on determination of the set of reliable genes for quantification of gene expression in pig. We tested six different genes across the tissues to determine the most stable reference genes for relative quantification of gene expression by real time RT-PCR. During the study we found out that only four genes from the set were suitable for other analyses. Thus, we used four genes EEF1A1, GAPDH, HPRT1, TOP2B for study of their stability across the pig tissues. By assistance of geNorm program we determined the most stable reference genes in muscle, liver and lungs. This information is useful for study of gene expression profiles in pigs. Práce je zaměřena na stanovení vhodných referenčních genů pro kvantifikaci genové exprese u prasat. Our work is focused on determination of the set of reliable genes for quantification of gene expression in pig. We tested six different genes across the tissues to determine the most stable reference genes for relative quantification of gene expression by real time RT-PCR. During the study we found out that only four genes from the set were suitable for other analyses. Thus, we used four genes EEF1A1, GAPDH, HPRT1, TOP2B for study of their stability across the pig tissues. By assistance of geNorm program we determined the most stable reference genes in muscle, liver and lungs. This information is useful for study of gene expression profiles in pigs.
dcterms:title
VALIDACE REFERENČNÍCH GENŮ PRO RELATIVNÍ KVANTIFIKACI GENOVÉ EXPRESE POMOCÍ REAL TIME RT-PCR Validation of reference genes for relative quantification of gene expression by real time rt-pcr Validation of reference genes for relative quantification of gene expression by real time rt-pcr
skos:prefLabel
Validation of reference genes for relative quantification of gene expression by real time rt-pcr VALIDACE REFERENČNÍCH GENŮ PRO RELATIVNÍ KVANTIFIKACI GENOVÉ EXPRESE POMOCÍ REAL TIME RT-PCR Validation of reference genes for relative quantification of gene expression by real time rt-pcr
skos:notation
RIV/62156489:43210/08:00118570!RIV08-MSM-43210___
n3:strany
373;375
n3:aktivita
n16:S
n3:aktivity
S
n3:dodaniDat
n4:2008
n3:domaciTvurceVysledku
n11:9075542 n11:7982054 n11:2529157
n3:druhVysledku
n20:D
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
402226
n3:idVysledku
RIV/62156489:43210/08:00118570
n3:jazykVysledku
n18:eng
n3:klicovaSlova
gene expression; pig; real time RT-PCR
n3:klicoveSlovo
n13:pig n13:real%20time%20RT-PCR n13:gene%20expression
n3:kontrolniKodProRIV
[1FD252FDA6DC]
n3:mistoKonaniAkce
České Budějovice
n3:mistoVydani
České Budějovice
n3:nazevZdroje
Biotechnology 2008
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n4:2008
n3:tvurceVysledku
Svobodová, Kateřina Knoll, Aleš Bílek, Karel
n3:typAkce
n8:EUR
n3:zahajeniAkce
2008-02-13+01:00
s:numberOfPages
3
n12:hasPublisher
Scientific Pedagogical Publishing
n17:isbn
80-85645-58-0
n7:organizacniJednotka
43210