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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F08%3A00114109%21RIV09-GA0-43210___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
We used suppression subtractive hybridization to search for genes more expressed in fetal hind limb muscles of Piétrain pigs compared to adult biceps femoris. The following genes were studied: FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS. By sequencing PCR-generated genomic fragments of a Piétrain and a Meishan pig, SNPs and indels were identified in all studied genes except for YWHAQ. Polymorphisms were tested by PCR-RFLP. Unrelated pigs of eight breeds (Large White, Landrace, Piétrain, Black Pied Prestice, Czech Meat Pig, Hampshire, Duroc and Meishan) were genotyped and allele frequencies are presented. Linkage mapping was performed in the Hohenheim Meishan x Piétrain (MxP) and/or Wild Boar x Meishan (WxM) three-generation pedigrees. RH mapping was performed using the porcine whole-genome 7000 rad IMpRH panel exept of CNN3 and POSTN. Použili jsme subtraktivní hybridizaci k výběru genů více exprimovaných ve svalovině plodu než dospělého jedince u prasat plemene pietrain. Byly studovány následující geny: FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 a GNAS. Byly identifikovány SNP a in/del polymorfizmy u většiny genů s vyjímkou YWHAQ. Polymorfizmy byly testovány PCR-RFLP a jsou prezentovány frekvence alel a genotypů. Bylo provedeno vyzbové a RH mapování. We used suppression subtractive hybridization to search for genes more expressed in fetal hind limb muscles of Piétrain pigs compared to adult biceps femoris. The following genes were studied: FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS. By sequencing PCR-generated genomic fragments of a Piétrain and a Meishan pig, SNPs and indels were identified in all studied genes except for YWHAQ. Polymorphisms were tested by PCR-RFLP. Unrelated pigs of eight breeds (Large White, Landrace, Piétrain, Black Pied Prestice, Czech Meat Pig, Hampshire, Duroc and Meishan) were genotyped and allele frequencies are presented. Linkage mapping was performed in the Hohenheim Meishan x Piétrain (MxP) and/or Wild Boar x Meishan (WxM) three-generation pedigrees. RH mapping was performed using the porcine whole-genome 7000 rad IMpRH panel exept of CNN3 and POSTN.
dcterms:title
Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles Mapování prasečích genů FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS, exprimovaných v kosterní svalovině plodu Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
skos:prefLabel
Mapování prasečích genů FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS, exprimovaných v kosterní svalovině plodu Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
skos:notation
RIV/62156489:43210/08:00114109!RIV09-GA0-43210___
n3:aktivita
n14:P
n3:aktivity
P(GA523/03/0858), P(GA523/06/1302)
n3:cisloPeriodika
2
n3:dodaniDat
n15:2009
n3:domaciTvurceVysledku
n7:9075542 n7:2529157 n7:7982054 n7:9635343
n3:druhVysledku
n18:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
377745
n3:idVysledku
RIV/62156489:43210/08:00114109
n3:jazykVysledku
n17:eng
n3:klicovaSlova
subtractive hybridization; polymorphism
n3:klicoveSlovo
n4:polymorphism n4:subtractive%20hybridization
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[E47E073A3E75]
n3:nazevZdroje
Animal Genetics
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
10
n3:projekt
n11:GA523%2F06%2F1302 n11:GA523%2F03%2F0858
n3:rokUplatneniVysledku
n15:2008
n3:svazekPeriodika
39
n3:tvurceVysledku
Horák, Pavel Stratil, Antonín Knoll, Aleš Peelman, Luck J. Bechyňová, R. Svobodová, Kateřina Van Poucke, Mario Geldermann, Hermann Bartenschlager, Heinz Bílek, Karel
s:issn
0268-9146
s:numberOfPages
2
n9:organizacniJednotka
43210