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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F07%3A00112571%21RIV08-AV0-43210___
rdf:type
skos:Concept n21:Vysledek
dcterms:description
Easy, efficient and low demanding separation of mRNA from biological material is needed to study gene expression and to use in chip technologies. To rapid and selective isolation of mRNA new approaches using modified surface of paramagnetic beads have been developed. It is common knowledge that each mRNA molecule contains sequence of 25 adenines. This feature can be used for binding of mRNA on the surface of the beads coated by thymine chains. The present work reports on suggesting and optimizing of mRNA separation and detection from biological material via paramagnetic beads coupled with electrochemical detection. Easy, efficient and low demanding separation of mRNA from biological material is needed to study gene expression and to use in chip technologies. To rapid and selective isolation of mRNA new approaches using modified surface of paramagnetic beads have been developed. It is common knowledge that each mRNA molecule contains sequence of 25 adenines. This feature can be used for binding of mRNA on the surface of the beads coated by thymine chains. The present work reports on suggesting and optimizing of mRNA separation and detection from biological material via paramagnetic beads coupled with electrochemical detection. Jednochá, účinná a nenáročná separace mRNA z bolofického materiálu je potřeba pro studium genové exprese a použití v čipových technologiích. Pro rychlou a selektivní izolaci mRNA byly vyvinuty nové přístupy s použitím paramagnetických kuliček s modifikovaným povrchem. Je známo, že každá molekula mRNA obsahuje sekvenci 25 adeninů, tato vlastnost může být použita pro navázání mRNA na povrch kuliček pokrytých řetězci tyminů. V této práci byla navrhnuta a optimalizována mRNA separace pomocí paramagnetických kuliček s elektrochemickou detekcí.
dcterms:title
Jednoduchá a rychlá separace a stanovení mRNA z biologických vzorků pomocí paramagnetických kuliček spojené s elektrochemickou detekcí Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection
skos:prefLabel
Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection Jednoduchá a rychlá separace a stanovení mRNA z biologických vzorků pomocí paramagnetických kuliček spojené s elektrochemickou detekcí
skos:notation
RIV/62156489:43210/07:00112571!RIV08-AV0-43210___
n4:strany
79;79
n4:aktivita
n7:P
n4:aktivity
P(IAA401990701)
n4:dodaniDat
n8:2008
n4:domaciTvurceVysledku
n6:8279314 n6:2104237 n6:4995775 n6:2307049 n6:9000240
n4:druhVysledku
n18:D
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
418594
n4:idVysledku
RIV/62156489:43210/07:00112571
n4:jazykVysledku
n16:eng
n4:klicovaSlova
Paramagnetic beads; mRNA; electrochemical detection
n4:klicoveSlovo
n9:electrochemical%20detection n9:Paramagnetic%20beads n9:mRNA
n4:kontrolniKodProRIV
[389C8FA423EE]
n4:mistoKonaniAkce
Kaunas, Lithuania
n4:nazevZdroje
4th Nordic Separation Science Society (NoSSS) International Conference
n4:obor
n20:CE
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
7
n4:projekt
n15:IAA401990701
n4:rokUplatneniVysledku
n8:2007
n4:tvurceVysledku
Horna, Aleš Húska, Dalibor Baloun, Jiří Trnková, Libuše Adam, Vojtěch Křížková, Soňa Kizek, René
n4:typAkce
n19:WRD
n4:zahajeniAkce
2007-01-01+01:00
s:numberOfPages
1
n14:hasPublisher
Neuveden
n13:isbn
978-9955-12-232-6
n11:organizacniJednotka
43210