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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F07%3A00107957%21RIV08-GA0-43210___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression level was found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provide a new knowled This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression level was found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provide a new knowled This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression level was found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provide a new knowled
dcterms:title
Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds
skos:prefLabel
Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds
skos:notation
RIV/62156489:43210/07:00107957!RIV08-GA0-43210___
n4:strany
78;83
n4:aktivita
n14:P
n4:aktivity
P(GA523/06/1302), P(GD523/03/H076)
n4:dodaniDat
n8:2008
n4:domaciTvurceVysledku
n16:9075542 n16:2529157 n16:7982054
n4:druhVysledku
n13:D
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
414376
n4:idVysledku
RIV/62156489:43210/07:00107957
n4:jazykVysledku
n7:eng
n4:klicovaSlova
real time PCR; gene expression; skeletal muscle
n4:klicoveSlovo
n10:skeletal%20muscle n10:gene%20expression n10:real%20time%20PCR
n4:kontrolniKodProRIV
[D43E0A0D0276]
n4:mistoKonaniAkce
Brno
n4:mistoVydani
Brno
n4:nazevZdroje
VIIth International conference of PhD. and MSc. students %22Genetics and Animal Breeding%22
n4:obor
n6:EB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n5:GA523%2F06%2F1302 n5:GD523%2F03%2FH076
n4:rokUplatneniVysledku
n8:2007
n4:tvurceVysledku
Bílek, Karel Knoll, Aleš Svobodová, Kateřina
n4:typAkce
n11:EUR
n4:zahajeniAkce
2007-05-17+02:00
s:numberOfPages
6
n21:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně. Ediční středisko
n19:isbn
978-80-7375-053-4
n20:organizacniJednotka
43210