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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F07%3A00099035%21RIV08-GA0-43210___
rdf:type
n16:Vysledek skos:Concept
dcterms:description
Dva polární benzo[c]fenanthridinové alkaloidy, chelerythrin (CHE) a dihydrochelerythrin (DHCHE), byly extrahovány při 35 °C a 10 MPa (15 MPa u reálných vzorků) z reálných a spajkovaných vzorků plasmy s přijatelnými návratnostmi (95.1 % and 81.0 %) pomocí superkritického oxidu uhličitého modifikovaného směsí (1:1, v/v) voda/methanol. Alkaloidy byly stanoveny HPLC/ESI-MS na koloně Zorbax SB-CN gradientovou elucí mobilní fázi methanol/50 mM mravenčan amonný. LOD byl 1.22 ng (3.50 pmol) pro CHE a 0.95 ng (2.72 pmol) pro DHCHE v 1 ml vzorku. Průměrná návratnost byla 100.1 % pro spajkovaný i reálný vzorek extraktů plasmy. Metodou byly extrahovány a analyzovány reálné vzorky plasmy potkanů. Two polar benzo[c]phenanthridine alkaloids, chelerythrine (CHE) and dihydrochelerythrine (DHCHE), were extracted at 35 °C and 10 MPa (15 MPa for real samples) from real and spiked plasma samples with acceptable recoveries (95.1 % and 81.0 %, respectively) using near-critical CO2 modified with aqueous (1:1, v/v) methanol. The alkaloids were quantified by a liquid chromatographic/electrospray mass spectrometric (LC/ESI-MS) method on a Zorbax SB-CN column (75 x 4.6 mm, 3.5 mm particle size) using methanol (organic phase) and 50 mM ammonium formiate (aqueous phase) as a mobile phase. A linear gradient 0 to 1 min - isocratic at 60% aqueous phase (v/v); from 1.0 to 7.0 min - 60% to 71% aqueous phase (v/v); from 7.0 to 18.0 min - 71% to 60% aqueous phase (v/v) was applied. The limit of detection was 1.22 ng (3.50 pmol) for CHE and 0.95 ng (2.72 pmol) for DHCHE per 1 ml of the sample. The linearity of the calibration curves was satisfactory as indicated by coefficients of determination 0.9979 and 0.9995 for C Two polar benzo[c]phenanthridine alkaloids, chelerythrine (CHE) and dihydrochelerythrine (DHCHE), were extracted at 35 °C and 10 MPa (15 MPa for real samples) from real and spiked plasma samples with acceptable recoveries (95.1 % and 81.0 %, respectively) using near-critical CO2 modified with aqueous (1:1, v/v) methanol. The alkaloids were quantified by a liquid chromatographic/electrospray mass spectrometric (LC/ESI-MS) method on a Zorbax SB-CN column (75 x 4.6 mm, 3.5 mm particle size) using methanol (organic phase) and 50 mM ammonium formiate (aqueous phase) as a mobile phase. A linear gradient 0 to 1 min - isocratic at 60% aqueous phase (v/v); from 1.0 to 7.0 min - 60% to 71% aqueous phase (v/v); from 7.0 to 18.0 min - 71% to 60% aqueous phase (v/v) was applied. The limit of detection was 1.22 ng (3.50 pmol) for CHE and 0.95 ng (2.72 pmol) for DHCHE per 1 ml of the sample. The linearity of the calibration curves was satisfactory as indicated by coefficients of determination 0.9979 and 0.9995 for C
dcterms:title
HPLC/ESI-MS stanovení sanguinarinu a chelerythrinu po extrakci superkritickým oxidem uhličitým zs spajkkovaných a reálných vzorků plasmy. High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples
skos:prefLabel
HPLC/ESI-MS stanovení sanguinarinu a chelerythrinu po extrakci superkritickým oxidem uhličitým zs spajkkovaných a reálných vzorků plasmy. High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples
skos:notation
RIV/62156489:43210/07:00099035!RIV08-GA0-43210___
n3:strany
1348;1356
n3:aktivita
n14:P
n3:aktivity
P(GA525/07/0871)
n3:cisloPeriodika
4
n3:dodaniDat
n11:2008
n3:domaciTvurceVysledku
n6:1150936 n6:8683913 n6:8905177
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
424078
n3:idVysledku
RIV/62156489:43210/07:00099035
n3:jazykVysledku
n10:eng
n3:klicovaSlova
HPLC; alkaloids; plasma samples
n3:klicoveSlovo
n9:plasma%20samples n9:alkaloids n9:HPLC
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[0AB513B48528]
n3:nazevZdroje
Talanta
n3:obor
n4:JU
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n13:GA525%2F07%2F0871
n3:rokUplatneniVysledku
n11:2007
n3:svazekPeriodika
72
n3:tvurceVysledku
Kubáň, Vlastimil Ulrichová, Jitka Lojková, Lea Klejdus, Bořivoj Šimánek, Vilím Kosina, Pavel
s:issn
0039-9140
s:numberOfPages
9
n12:organizacniJednotka
43210