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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F06%3A00099021%21RIV07-GA0-43210___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Genetic variability was detected in 15 varieties of triticale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR) registered in the Czech Republic by means of polymorphism of DNA using the RAPD method and the SSR method. For detection we used 80 RAPD primers. The lower reproducibility of the RAPD markers was resolved by means of repeated analyses (3--4 times). On the basis of statistical evaluation a dendrogram was set up, which allows highly significantly to differentiate the varieties Kolor, Modus and Tornado. The remaining 12 analysed varieties formed 4 clusters. In addition to the RAPD markers a protocol of detection of DNA polymorphism was elaborated and optimised with microsatellite (SSR) markers. For the analyses we used 2 SSR markers (1A chromosome [Xpsp2999] and 1B chromosome [Xpsp3000]), which have been discovered in wheat (T. aestivum L.). Basing on these two SSR markers the only variety Triamant was distinguished from the clusters of the other analysed varieties. Genetic variability was detected in 15 varieties of triticale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR) registered in the Czech Republic by means of polymorphism of DNA using the RAPD method and the SSR method. For detection we used 80 RAPD primers. The lower reproducibility of the RAPD markers was resolved by means of repeated analyses (3--4 times). On the basis of statistical evaluation a dendrogram was set up, which allows highly significantly to differentiate the varieties Kolor, Modus and Tornado. The remaining 12 analysed varieties formed 4 clusters. In addition to the RAPD markers a protocol of detection of DNA polymorphism was elaborated and optimised with microsatellite (SSR) markers. For the analyses we used 2 SSR markers (1A chromosome [Xpsp2999] and 1B chromosome [Xpsp3000]), which have been discovered in wheat (T. aestivum L.). Basing on these two SSR markers the only variety Triamant was distinguished from the clusters of the other analysed varieties. Genetická variabilita byla detekována metodami molekulární genetiky (RAPD a SSR) u 15 odrůd tritikale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR), které jsou registrovány v České republice. Pro detekci polymorfizmu DNA bylo využito 80 RAPD primerů. Nižší reprodukovatelnost RAPD analýz byla kompenzována opakováním analýz (3--4x). Na základě statistického vyhodnocení byl sestaven dendrogram, kde je možno vysoce průkazně odlišit odrůdy Kolor, Modus a Tornado. Zbývajících 12 analyzovaných odrůd tvoří čtyři klastery. Byl optimalizován protokol detekce variability DNA pomocí mikrosatelitních markerů u tritikale. Pro analýzy byly využity dva SSR markery (1A chromozom [Xpsp2999] a 1B chromozom [Xpsp3000]), které byly popsány u pšenice obecné (T. aestivum L.). Na základě použitých dvou SSR markerů se podařilo odlišit odrůdu Triamant od klasterů ostatních analyzovaných odrůd.
dcterms:title
Molekulární markery genetické variability u odrůd tritikale registrovaných v České republice Molecular markers of genetic variability in triticale varieties registered in the Czech Republic Molecular markers of genetic variability in triticale varieties registered in the Czech Republic
skos:prefLabel
Molecular markers of genetic variability in triticale varieties registered in the Czech Republic Molekulární markery genetické variability u odrůd tritikale registrovaných v České republice Molecular markers of genetic variability in triticale varieties registered in the Czech Republic
skos:notation
RIV/62156489:43210/06:00099021!RIV07-GA0-43210___
n3:strany
149;154
n3:aktivita
n11:P
n3:aktivity
P(GP521/03/P173)
n3:cisloPeriodika
5
n3:dodaniDat
n16:2007
n3:domaciTvurceVysledku
n4:6512348 n4:9220542
n3:druhVysledku
n13:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
486619
n3:idVysledku
RIV/62156489:43210/06:00099021
n3:jazykVysledku
n18:eng
n3:klicovaSlova
RAPD; SSR; triticale
n3:klicoveSlovo
n8:RAPD n8:SSR n8:triticale
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[892B2CF2C9CF]
n3:nazevZdroje
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n12:GP521%2F03%2FP173
n3:rokUplatneniVysledku
n16:2006
n3:svazekPeriodika
LIV
n3:tvurceVysledku
Vyhnánek, Tomáš Bednář, Jan
s:issn
1211-8516
s:numberOfPages
6
n17:organizacniJednotka
43210