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Statements

Subject Item
n2:RIV%2F62156489%3A43210%2F05%3A00004744%21RIV06-GA0-43210___
rdf:type
n15:Vysledek skos:Concept
dcterms:description
We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 microM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 microM of 2,4-D and 4 microM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 microM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 microM)conntributed to their rapid conversion to plantlets. The article is in English. See original desription in the original language. We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 microM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 microM of 2,4-D and 4 microM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 microM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 microM)conntributed to their rapid conversion to plantlets.
dcterms:title
Somatic embryogenesis from zygotic embryos of Schisandra chinensis Somatic embryogenesis from zygotic embryos of Schisandra chinensis Somatic embryogenesis from zygotic embryos of Schisandra chinensis
skos:prefLabel
Somatic embryogenesis from zygotic embryos of Schisandra chinensis Somatic embryogenesis from zygotic embryos of Schisandra chinensis Somatic embryogenesis from zygotic embryos of Schisandra chinensis
skos:notation
RIV/62156489:43210/05:00004744!RIV06-GA0-43210___
n5:strany
451;454
n5:aktivita
n18:P
n5:aktivity
P(GA521/02/1129)
n5:cisloPeriodika
3
n5:dodaniDat
n12:2006
n5:domaciTvurceVysledku
n14:2453290 n14:3036537 n14:1249940
n5:druhVysledku
n16:J
n5:duvernostUdaju
n6:S
n5:entitaPredkladatele
n13:predkladatel
n5:idSjednocenehoVysledku
543480
n5:idVysledku
RIV/62156489:43210/05:00004744
n5:jazykVysledku
n8:cze
n5:klicovaSlova
abscisic acied; maturation; medicinal plant; micropropagation; somatic embryo conversion
n5:klicoveSlovo
n7:somatic%20embryo%20conversion n7:abscisic%20acied n7:micropropagation n7:medicinal%20plant n7:maturation
n5:kodStatuVydavatele
CZ - Česká republika
n5:kontrolniKodProRIV
[0B5186E38F21]
n5:nazevZdroje
Biologia Plantarum
n5:obor
n17:AA
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
3
n5:projekt
n11:GA521%2F02%2F1129
n5:rokUplatneniVysledku
n12:2005
n5:svazekPeriodika
49
n5:tvurceVysledku
Havel, Ladislav Vlašínová, Helena Smíšková, Alexandra
s:issn
0006-3134
s:numberOfPages
4
n10:organizacniJednotka
43210