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Statements

Subject Item
n2:RIV%2F61989592%3A15410%2F03%3A00001414%21RIV%2F2004%2FMSM%2F154104%2FN
rdf:type
n9:Vysledek skos:Concept
dcterms:description
HPLC and CE methods were employed to determine the quaternary benzo[c]phenanthridine alkaloid sanguinarine,in biological matrices (rat hepatocytes, human gingival fibroblasts, feed, porcine faeces, body fluids and tissues). HPLC was carried out on a C-18column using gradient elution and ion pairing techniques with 1-heptanesulfonic acid as ion pairing agent under acidic conditions. The detection limit for fluorimetric detection at lambda(ex) = 327 nm and lambda(em) = 577 nm was 3 nM sanguinarine. CE an alyses were performed in 50 mM phosphate-Na buffer pH 2.5, with 150 mM SDS used for pre-concentration by the sweeping effect. This experimental configuration allows injecting the total capillary length with sanguinarine sample. The detection limit for UVdetection at 285 nm was 12 nM. Both methods are suitable for analysing submicromolar quantities of sanguinarine in biological materials. The HPLC method is more sensitive than CE because it uses fluorescence detection. HPLC and CE methods were employed to determine the quaternary benzo[c]phenanthridine alkaloid sanguinarine,in biological matrices (rat hepatocytes, human gingival fibroblasts, feed, porcine faeces, body fluids and tissues). HPLC was carried out on a C-18column using gradient elution and ion pairing techniques with 1-heptanesulfonic acid as ion pairing agent under acidic conditions. The detection limit for fluorimetric detection at lambda(ex) = 327 nm and lambda(em) = 577 nm was 3 nM sanguinarine. CE an alyses were performed in 50 mM phosphate-Na buffer pH 2.5, with 150 mM SDS used for pre-concentration by the sweeping effect. This experimental configuration allows injecting the total capillary length with sanguinarine sample. The detection limit for UVdetection at 285 nm was 12 nM. Both methods are suitable for analysing submicromolar quantities of sanguinarine in biological materials. The HPLC method is more sensitive than CE because it uses fluorescence detection.
dcterms:title
High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices
skos:prefLabel
High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices High performance liquid chromatography and capillary electrophoresis determination of sanguinarine in biological matrices
skos:notation
RIV/61989592:15410/03:00001414!RIV/2004/MSM/154104/N
n3:strany
679-685
n3:aktivita
n7:Z
n3:aktivity
Z(MSM 151100003), Z(MSM 153100013)
n3:cisloPeriodika
8
n3:dodaniDat
n11:2004
n3:domaciTvurceVysledku
n10:8341362 n10:2514559 n10:1030957 n10:6267408 n10:1089935 n10:1077074 n10:8390924
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
608799
n3:idVysledku
RIV/61989592:15410/03:00001414
n3:jazykVysledku
n12:eng
n3:klicovaSlova
SAMPLE SELF-STACKING; ZONE-ELECTROPHORESIS; BENZOPHENANTHRIDINE ALKALOIDS; FLUOROMETRIC DETECTION; SEPARATION; ISOTACHOPHORESIS; CHARGE; HPLC
n3:klicoveSlovo
n4:BENZOPHENANTHRIDINE%20ALKALOIDS n4:HPLC n4:SEPARATION n4:ISOTACHOPHORESIS n4:FLUOROMETRIC%20DETECTION n4:SAMPLE%20SELF-STACKING n4:CHARGE n4:ZONE-ELECTROPHORESIS
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[9DE9389181F5]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n5:CB
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
8
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:rokUplatneniVysledku
n11:2003
n3:svazekPeriodika
26
n3:tvurceVysledku
Ulrichová, Jitka Bednář, Petr Kosina, Pavel Ševčík, Juraj Gavenda, A. Walterová, Daniela Barták, Petr Modrianský, Martin
n3:zamer
n13:MSM%20151100003 n13:MSM%20153100013
s:issn
1615-9306
s:numberOfPages
7
n18:organizacniJednotka
15410