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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F14%3A33151716%21RIV15-MSM-15310___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://link.springer.com/article/10.1007%2Fs00344-014-9419-z
dcterms:description
Our knowledge on the principle mechanisms of cytokinin action has been significantly deepened over the last years, but several weakly explored areas still remain on the map of cytokinin cellular physiology. Cytokinin-binding proteins could also be included in this pending field of cytokinin research. Probably, the best explored representative of this group is the wheat cytokinin-binding protein 1 (CBP-1). The role of this germ-allocated protein as a presumable regulator of free aromatic cytokinin levels during grain germination has been discussed intensively. To dig deeper into this interesting protein, this study was aimed at the identification of the CBP-1 amino acid sequence. A combination of in silico BLAST search, classical biochemical CBP-1 purification based on isoelectric point precipitation and ion-exchange chromatography, and proteomic analysis of the isolated protein by ultra-high resolution tandem mass spectrometry allowed us to uncover and validate two CBP-1 subunit candidate sequences with molecular masses of 56.2 and 55.0 kDa, respectively. Interestingly, we found the latter sequence alternated in two amino acids in the putative cytokinin-binding motive in comparison to the composition of this domain reported in the original studies. A BLAST search for the amino acid sequence of the binding region among plant proteomes revealed several highly related protein sequences, all originating from the Poaceae family. This piece of information could give support to the elucidation of the role of CBP-1 in physiological processes mediated by aromatic cytokinins. Our knowledge on the principle mechanisms of cytokinin action has been significantly deepened over the last years, but several weakly explored areas still remain on the map of cytokinin cellular physiology. Cytokinin-binding proteins could also be included in this pending field of cytokinin research. Probably, the best explored representative of this group is the wheat cytokinin-binding protein 1 (CBP-1). The role of this germ-allocated protein as a presumable regulator of free aromatic cytokinin levels during grain germination has been discussed intensively. To dig deeper into this interesting protein, this study was aimed at the identification of the CBP-1 amino acid sequence. A combination of in silico BLAST search, classical biochemical CBP-1 purification based on isoelectric point precipitation and ion-exchange chromatography, and proteomic analysis of the isolated protein by ultra-high resolution tandem mass spectrometry allowed us to uncover and validate two CBP-1 subunit candidate sequences with molecular masses of 56.2 and 55.0 kDa, respectively. Interestingly, we found the latter sequence alternated in two amino acids in the putative cytokinin-binding motive in comparison to the composition of this domain reported in the original studies. A BLAST search for the amino acid sequence of the binding region among plant proteomes revealed several highly related protein sequences, all originating from the Poaceae family. This piece of information could give support to the elucidation of the role of CBP-1 in physiological processes mediated by aromatic cytokinins.
dcterms:title
Proteomic identification of a candidate sequence of wheat cytokinin-binding protein 1 Proteomic identification of a candidate sequence of wheat cytokinin-binding protein 1
skos:prefLabel
Proteomic identification of a candidate sequence of wheat cytokinin-binding protein 1 Proteomic identification of a candidate sequence of wheat cytokinin-binding protein 1
skos:notation
RIV/61989592:15310/14:33151716!RIV15-MSM-15310___
n3:aktivita
n16:P
n3:aktivity
P(LO1204)
n3:cisloPeriodika
4
n3:dodaniDat
n12:2015
n3:domaciTvurceVysledku
n15:7740964 n15:9896821 n15:3719332 n15:9708790 n15:4712048 n15:9685251
n3:druhVysledku
n13:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
40491
n3:idVysledku
RIV/61989592:15310/14:33151716
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Germination; Mass spectrometry; Identification; Proteomics; Amino acid sequence; Cytokinin-binding protein; Cytokinin
n3:klicoveSlovo
n4:Mass%20spectrometry n4:Germination n4:Cytokinin-binding%20protein n4:Amino%20acid%20sequence n4:Cytokinin n4:Identification n4:Proteomics
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[0BDD1C05B17C]
n3:nazevZdroje
Journal of Plant Growth Regulation
n3:obor
n9:CE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n7:LO1204
n3:rokUplatneniVysledku
n12:2014
n3:svazekPeriodika
33
n3:tvurceVysledku
Béresová, Lucie Lenobel, René Šebela, Marek Chamrád, Ivo Strnad, Miroslav Simerský, Radim
n3:wos
000344917800018
s:issn
0721-7595
s:numberOfPages
7
n10:doi
10.1007/s00344-014-9419-z
n11:organizacniJednotka
15310