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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F14%3A33151668%21RIV15-MSM-15310___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0734975013001948
dcterms:description
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a reliable tool for fast identification and classification of microorganisms. In this regard, it represents a strong challenge to microscopic and molecular biology methods. Nowadays, commercial MALDI systems are accessible for biological research work as well as for diagnostic applications in clinical medicine, biotechnology and industry. They are employed namely in bacterial biotyping but numerous experimental strategies have also been developed for the analysis of fungi, which is the topic of the present review. Members of many fungal genera such as Aspergillus, Fusarium, Penicillium or Trichoderma and also various yeasts from clinical samples (e.g. Candida albicans) have been successfully identified by MALDI-TOF MS. However, there is no versatile method for fungi currently available even though the use of only a limited number of matrix compounds has been reported. Either intact cell/spore MALDI-TOF MS is chosen or an extraction of surface proteins is performed and then the resulting extract is measured. Biotrophic fungal phytopathogens can be identified via a direct acquisition of MALDI-TOF mass spectra e.g. from infected plant organs contaminated by fungal spores. Mass spectrometric peptide/protein profiles of fungi display peaks in the m/z region of 1000-20 000, where a unique set of biomarker ions may appear facilitating a differentiation of samples at the level of genus, species or strain. This is done with the help of a processing software and spectral database of reference strains, which should preferably be constructed under the same standardized experimental conditions. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a reliable tool for fast identification and classification of microorganisms. In this regard, it represents a strong challenge to microscopic and molecular biology methods. Nowadays, commercial MALDI systems are accessible for biological research work as well as for diagnostic applications in clinical medicine, biotechnology and industry. They are employed namely in bacterial biotyping but numerous experimental strategies have also been developed for the analysis of fungi, which is the topic of the present review. Members of many fungal genera such as Aspergillus, Fusarium, Penicillium or Trichoderma and also various yeasts from clinical samples (e.g. Candida albicans) have been successfully identified by MALDI-TOF MS. However, there is no versatile method for fungi currently available even though the use of only a limited number of matrix compounds has been reported. Either intact cell/spore MALDI-TOF MS is chosen or an extraction of surface proteins is performed and then the resulting extract is measured. Biotrophic fungal phytopathogens can be identified via a direct acquisition of MALDI-TOF mass spectra e.g. from infected plant organs contaminated by fungal spores. Mass spectrometric peptide/protein profiles of fungi display peaks in the m/z region of 1000-20 000, where a unique set of biomarker ions may appear facilitating a differentiation of samples at the level of genus, species or strain. This is done with the help of a processing software and spectral database of reference strains, which should preferably be constructed under the same standardized experimental conditions.
dcterms:title
Identification of fungal microorganisms by MALDI-TOF mass spectrometry Identification of fungal microorganisms by MALDI-TOF mass spectrometry
skos:prefLabel
Identification of fungal microorganisms by MALDI-TOF mass spectrometry Identification of fungal microorganisms by MALDI-TOF mass spectrometry
skos:notation
RIV/61989592:15310/14:33151668!RIV15-MSM-15310___
n3:aktivita
n14:P
n3:aktivity
P(7AMB12AT018), P(ED0007/01/01), P(EE2.4.31.0130)
n3:cisloPeriodika
1
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n4:2879352 n4:5464900 n4:9708790 n4:1301411
n3:druhVysledku
n7:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
20283
n3:idVysledku
RIV/61989592:15310/14:33151668
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Yeasts; Taxonomy; Protein; Peptide; Mildew; MALDI; Intact cell/spore mass spectrometry; Identification; Fungi; Biotyping
n3:klicoveSlovo
n6:Mildew n6:Protein n6:Taxonomy n6:Intact%20cell%2Fspore%20mass%20spectrometry n6:MALDI n6:Yeasts n6:Identification n6:Biotyping n6:Fungi n6:Peptide
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[472D4B72A90E]
n3:nazevZdroje
Biotechnology Advances
n3:obor
n9:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n5:EE2.4.31.0130 n5:7AMB12AT018 n5:ED0007%2F01%2F01
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
32
n3:tvurceVysledku
Sedlářová, Michaela Chalupová, Jana Raus, Martin Šebela, Marek
n3:wos
000332051500018
s:issn
0734-9750
s:numberOfPages
12
n12:doi
10.1016/j.biotechadv.2013.11.002
n19:organizacniJednotka
15310