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Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F14%3A33151655%21RIV15-MSM-15310___
rdf:type
n8:Vysledek skos:Concept
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400369/pdf
dcterms:description
Online restricted access media with liquid chromatography and tandem mass spectrometry for the direct analysis of small molecules in biological fluids represents an interesting alternative to time-demanding traditional sample preparation techniques. In this study, important considerations concerning the development of a restricted access media with liquid chromatography and tandem mass spectrometry method for the analysis of dansylated estrogens in biological matrix are presented. Parameters influencing peak tailing and trapping efficiency were evaluated. The key factors included the ion strength of the mobile phase, a loading flow rate of the sample onto the trap column, and selection of a proper stationary phase of the trap column for a given set of analytes. These parameters have proven to be essential for minimizing any unwanted chromatographic peak tailing. The bulk derivatization of the analytes in the biological fluids and its relationship to the observed matrix effects was evaluated as well. Online restricted access media with liquid chromatography and tandem mass spectrometry for the direct analysis of small molecules in biological fluids represents an interesting alternative to time-demanding traditional sample preparation techniques. In this study, important considerations concerning the development of a restricted access media with liquid chromatography and tandem mass spectrometry method for the analysis of dansylated estrogens in biological matrix are presented. Parameters influencing peak tailing and trapping efficiency were evaluated. The key factors included the ion strength of the mobile phase, a loading flow rate of the sample onto the trap column, and selection of a proper stationary phase of the trap column for a given set of analytes. These parameters have proven to be essential for minimizing any unwanted chromatographic peak tailing. The bulk derivatization of the analytes in the biological fluids and its relationship to the observed matrix effects was evaluated as well.
dcterms:title
Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method
skos:prefLabel
Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method
skos:notation
RIV/61989592:15310/14:33151655!RIV15-MSM-15310___
n4:aktivita
n7:P
n4:aktivity
P(ED2.1.00/03.0058), P(EE2.3.20.0058)
n4:cisloPeriodika
16
n4:dodaniDat
n11:2015
n4:domaciTvurceVysledku
n5:4316851 n5:3649768
n4:druhVysledku
n20:J
n4:duvernostUdaju
n13:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
4197
n4:idVysledku
RIV/61989592:15310/14:33151655
n4:jazykVysledku
n19:eng
n4:klicovaSlova
On-line sample preparation; Estrogens; Estradiol quantification; Bulk derivatization; Biological fluids
n4:klicoveSlovo
n14:Bulk%20derivatization n14:Estradiol%20quantification n14:Biological%20fluids n14:Estrogens n14:On-line%20sample%20preparation
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[211E400878CE]
n4:nazevZdroje
Journal of Separation Science
n4:obor
n12:CB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:projekt
n16:EE2.3.20.0058 n16:ED2.1.00%2F03.0058
n4:rokUplatneniVysledku
n11:2014
n4:svazekPeriodika
37
n4:tvurceVysledku
Lemr, Karel Schug, Kevin A Fan, Hui Papoušková, Barbora
n4:wos
000340556300014
s:issn
1615-9306
s:numberOfPages
8
n3:doi
10.1002/jssc.201400369
n15:organizacniJednotka
15310