This HTML5 document contains 60 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n16http://localhost/temp/predkladatel/
n14http://linked.opendata.cz/resource/domain/vavai/projekt/
n8http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n5http://linked.opendata.cz/ontology/domain/vavai/
n11http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61989592%3A15310%2F14%3A33150521%21RIV15-TA0-15310___/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n15http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n19http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n20http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61989592%3A15310%2F14%3A33150521%21RIV15-TA0-15310___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://pubs.acs.org/doi/pdf/10.1021/ac503347h
dcterms:description
Development of methods allowing determination of even ultralow levels of immunoglobulins in various clinical samples including whole human blood and plasma is a particular scientific challenge, especially due to many essential discoveries in the fields of immunology and medicine in the past few decades. The determination of IgG is usually performed using an enzymatic approach, followed by colorimetric or fluorimetric detection. However, limitations of these methods relate to their complicated setup and stringent requirements concerning the sample purity. Here, we present a novel approach based on magnetically assisted surface enhanced Raman spectroscopy (MA/SERS), which utilizes a Fe3O4@Ag@streptavidin@anti-IgG nanocomposite with strong magnetic properties and an efficient SERS enhancement factor conferred by the Fe3O4 particles and silver nanoparticles, respectively. Such a nanocomposite offers the possibility of separating a target efficiently from a complex matrix by simple application of an external magnetic force, followed by direct determination using SERS. High selectivity was achieved by the presence of anti-IgG on the surface of silver nanoparticles coupled with their further inactivation by ethylamine. Compared to many recently developed sandwich methods, application of single nanocomposites showed many advantages, including simplicity of use, direct control of the analytic process, and elimination of errors caused by possible nonspecific interactions. Moreover, incorporation of advanced spectral processing methods led to a considerable decrease in the relative error of determination to below 5%. Development of methods allowing determination of even ultralow levels of immunoglobulins in various clinical samples including whole human blood and plasma is a particular scientific challenge, especially due to many essential discoveries in the fields of immunology and medicine in the past few decades. The determination of IgG is usually performed using an enzymatic approach, followed by colorimetric or fluorimetric detection. However, limitations of these methods relate to their complicated setup and stringent requirements concerning the sample purity. Here, we present a novel approach based on magnetically assisted surface enhanced Raman spectroscopy (MA/SERS), which utilizes a Fe3O4@Ag@streptavidin@anti-IgG nanocomposite with strong magnetic properties and an efficient SERS enhancement factor conferred by the Fe3O4 particles and silver nanoparticles, respectively. Such a nanocomposite offers the possibility of separating a target efficiently from a complex matrix by simple application of an external magnetic force, followed by direct determination using SERS. High selectivity was achieved by the presence of anti-IgG on the surface of silver nanoparticles coupled with their further inactivation by ethylamine. Compared to many recently developed sandwich methods, application of single nanocomposites showed many advantages, including simplicity of use, direct control of the analytic process, and elimination of errors caused by possible nonspecific interactions. Moreover, incorporation of advanced spectral processing methods led to a considerable decrease in the relative error of determination to below 5%.
dcterms:title
Magnetically-Assisted Surface Enhanced Raman Spectroscopy (MA-SERS) for Label-Free Determination of Human Immunoglobulin G (IgG) in Blood Using Fe3O4@Ag Nanocomposite Magnetically-Assisted Surface Enhanced Raman Spectroscopy (MA-SERS) for Label-Free Determination of Human Immunoglobulin G (IgG) in Blood Using Fe3O4@Ag Nanocomposite
skos:prefLabel
Magnetically-Assisted Surface Enhanced Raman Spectroscopy (MA-SERS) for Label-Free Determination of Human Immunoglobulin G (IgG) in Blood Using Fe3O4@Ag Nanocomposite Magnetically-Assisted Surface Enhanced Raman Spectroscopy (MA-SERS) for Label-Free Determination of Human Immunoglobulin G (IgG) in Blood Using Fe3O4@Ag Nanocomposite
skos:notation
RIV/61989592:15310/14:33150521!RIV15-TA0-15310___
n3:aktivita
n9:S n9:P
n3:aktivity
P(ED2.1.00/03.0058), P(ED3.1.00/14.0302), P(EE2.3.20.0056), P(EE2.3.20.0155), P(TA03011368), S
n3:cisloPeriodika
22
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n8:8441731 n8:5994047 n8:5341620 n8:9251189 n8:6833446
n3:druhVysledku
n20:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
26917
n3:idVysledku
RIV/61989592:15310/14:33150521
n3:jazykVysledku
n19:eng
n3:klicovaSlova
multiplex; separation; flourescence; core; assay; protein; immunoassay; immunogold nanoparticles; scattering SERS; gold nanoparticles
n3:klicoveSlovo
n4:immunogold%20nanoparticles n4:scattering%20SERS n4:immunoassay n4:gold%20nanoparticles n4:separation n4:multiplex n4:assay n4:core n4:flourescence n4:protein
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[51023457468D]
n3:nazevZdroje
Analytical Chemistry
n3:obor
n18:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n14:ED2.1.00%2F03.0058 n14:EE2.3.20.0056 n14:ED3.1.00%2F14.0302 n14:EE2.3.20.0155 n14:TA03011368
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
86
n3:tvurceVysledku
Marková, Zdenka Ranc, Václav Zbořil, Radek Fargašová, Ariana Balzerová, Anna
n3:wos
000345263300020
s:issn
0003-2700
s:numberOfPages
8
n15:doi
10.1021/ac503347h
n16:organizacniJednotka
15310